End-binding protein 1 (EB1) up-regulation is an early event in colorectal carcinogenesis

•EB1 expression is significantly increased in early and field carcinogenesis in the colon.•Knockdown of EB1 reduced the cancerous phenotype in colon cancer cell lines.•LEBS analysis of EB1 knockdown cells shows distinct changes in cell nano-architecture. End-binding protein (EB1) is a microtubule pr...

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Bibliographic Details
Published in:FEBS letters 2014-03, Vol.588 (5), p.829-835
Main Authors: Stypula-Cyrus, Yolanda, Mutyal, Nikhil N., Dela Cruz, Mart, Kunte, Dhananjay P., Radosevich, Andrew J., Wali, Ramesh, Roy, Hemant K., Backman, Vadim
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Adenoma - genetics
Adenoma - metabolism
Adenoma - pathology
Adenomatous polyposis coli
Animals
Apoptosis
Carcinogenesis - metabolism
Cell Proliferation
Colon cancer
Colorectal Neoplasms - genetics
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - pathology
Cytoskeleton - metabolism
End-binding protein 1
Field carcinogenesis
Gene Expression Regulation, Neoplastic
HCT116 Cells
HT29 Cells
Humans
Male
Microtubule-Associated Proteins - genetics
Microtubule-Associated Proteins - metabolism
Rats
Sub-diffractional structure
Tissue Array Analysis
Up-Regulation
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Summary:•EB1 expression is significantly increased in early and field carcinogenesis in the colon.•Knockdown of EB1 reduced the cancerous phenotype in colon cancer cell lines.•LEBS analysis of EB1 knockdown cells shows distinct changes in cell nano-architecture. End-binding protein (EB1) is a microtubule protein that binds to the tumor suppressor adenomatous polyposis coli (APC). While EB1 is implicated as a potential oncogene, its role in cancer progression is unknown. Therefore, we analyzed EB1/APC expression at the earliest stages of colorectal carcinogenesis and in the uninvolved mucosa (“field effect”) of human and animal tissue. We also performed siRNA-knockdown in colon cancer cell lines. EB1 is up-regulated in early and field carcinogenesis in the colon, and the cellular/nano-architectural effect of EB1 knockdown depended on the genetic context. Thus, dysregulation of EB1 is an important early event in colon carcinogenesis.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2014.01.046