Survival and synergistic growth of mixed cultures of bifidobacteria and lactobacilli combined with prebiotic oligosaccharides in a gastrointestinal tract simulator

Probiotics, especially in combination with non-digestible oligosaccharides, may balance the gut microflora while multistrain preparations may express an improved functionality over single strain cultures. In vitro gastrointestinal models enable to test survival and growth dynamics of mixed strain pr...

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Bibliographic Details
Published in:Journal of aesthetics & culture 2014, Vol.25 (1)
Main Authors: Adamberg, Signe, Sumeri, Ingrid, Uusna, Riin, Ambalam, Padma, Kondepudi, Kanthi Kiran, Adamberg, Kaarel, Wadström, Torkel, Ljungh, Åsa
Format: Article
Language:English
Subjects:
Bacteria
Basic Medicine
Bifidobacterium
Bifidobacterium breve
Bile
Colleges & universities
Colon
Fermentation
Food
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gastrointestinal tract simulator
Inflammatory bowel disease
Influence
Lactobacillus
Lactobacillus plantarum
Medical and Health Sciences
Medicin och hälsovetenskap
Medicinska och farmaceutiska grundvetenskaper
Metabolism
Microbiology in the medical area
Microorganisms
Mikrobiologi inom det medicinska området
mixed culture
Molecular weight
Original
prebiotic oligosaccharides
Probiotics
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Summary:Probiotics, especially in combination with non-digestible oligosaccharides, may balance the gut microflora while multistrain preparations may express an improved functionality over single strain cultures. In vitro gastrointestinal models enable to test survival and growth dynamics of mixed strain probiotics in a controlled, replicable manner. The robustness and compatibility of multistrain probiotics composed of bifidobacteria and lactobacilli combined with mixed prebiotics (galacto-, fructo- and xylo-oligosaccharides or galactooligosaccharides and soluble starch) were studied using a dynamic gastrointestinal tract simulator (GITS). The exposure to acid and bile of the upper gastrointestinal tract was followed by dilution with a continuous decrease of the dilution rate (de-celerostat) to simulate the descending nutrient availability of the large intestine. The bacterial numbers and metabolic products were analyzed and the growth parameters determined. The most acid- and bile-resistant strains were Lactobacillus plantarum F44 and L. paracasei F8. Bifidobacterium breve 46 had the highest specific growth rate and, although sensitive to bile exposure, recovered during the dilution phase in most experiments. B. breve 46, L. plantarum F44, and L. paracasei F8 were selected as the most promising strains for further studies. De-celerostat cultivation can be applied to study the mixed bacterial cultures under defined conditions of decreasing nutrient availability to select a compatible set of strains.
ISSN:0891-060X
1651-2235
1651-2235
1455-9145
2000-4214
DOI:10.3402/mehd.v25.23062