Exercise ameliorates the detrimental effect of chloroquine on skeletal muscles in mice via restoring autophagy flux

Aim： To study the roles of autophagy in muscle establishment during long-term exercise in mice. Methods： Female ICR mice were submitted to exercise training with a wheel running regimen： 6 m/min, 15 min/time, 3 times/d （on 8：00, 14：00, and 20：00）, 5 d/week for 2 months. The mice were treated with th...

Full description

Saved in:
Bibliographic Details
Published in:Acta pharmacologica Sinica 2014-01, Vol.35 (1), p.135-142
Main Authors: Jiang, Dan, Chen, Kai, Lu, Xuan, Gao, Hong-jian, Qin, Zheng-hong, Lin, Fang
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Autophagy - drug effects
Autophagy - physiology
Chloroquine - toxicity
Female
ICR小鼠
Mice
Mice, Inbred ICR
Muscle, Skeletal - drug effects
Muscle, Skeletal - metabolism
Muscle, Skeletal - pathology
Original
Physical Conditioning, Animal - methods
Physical Conditioning, Animal - physiology
Quadriceps Muscle - drug effects
Quadriceps Muscle - metabolism
Quadriceps Muscle - pathology
Western印迹
氯喹
细胞质
自噬作用
运动训练
通量
骨骼肌
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aim： To study the roles of autophagy in muscle establishment during long-term exercise in mice. Methods： Female ICR mice were submitted to exercise training with a wheel running regimen： 6 m/min, 15 min/time, 3 times/d （on 8：00, 14：00, and 20：00）, 5 d/week for 2 months. The mice were treated with the autophagy activator trehalose （1% aqueous solution as a daily drinking water） or the autophagy inhibitor chloroquine （10 mg/kg, ip, 5 times a week） before the training. Western blotting analysis, TUNEL staining, H＆E staining and transmission electron microscope were used to evaluate the activity of autophagy and the structure of the muscle fibers. Results： The exercise training significantly stimulated the formation of autophagosomes, increased the LC3-II, cathepsin L and Bcl-2 levels, lowered the P62 level and increased the antioxidant capacity in the muscles. Meanwhile, the exercise training significantly improved the morphology of mitochondria, reduced the release of cytochrome c from mitochondria to cytoplasm, and slightly decreased the apoptosis rate in the muscles. Administration of trehalose increased the level of autophagy and protected the muscle fibers from apoptosis. Administration of chloroquine blocked autophagy flux and exerted detrimental effects on the muscles, which were ameliorated by the exercise training. Conclusion： Long-term regular exercise activates autophagy process associated with muscle establishment, and ameliorates the detrimental effects of chloroquine on skeletal muscles via restoring autophagy flux.
ISSN:1671-4083
1745-7254
DOI:10.1038/aps.2013.144