Stability studies of common biochemical analytes in serum separator tubes with or without gel barrier subjected to various storage conditions

The collected and shipped blood samples are exposed to a various extra-analytical factors prior to analysis. The aim of the study was to determine the stability of analytes in serum gel tubes and plain tubes exposed to a range of storage temperatures and times after centrifugation. Fifteen healthy v...

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Bibliographic Details
Published in:Biochemia medica 2012-01, Vol.22 (2), p.202-214
Main Authors: Cuhadar, Serap, Atay, Ayşenur, Koseoglu, Mehmet, Dirican, Ahmet, Hur, Aysel
Format: Article
Language:English
Subjects:
Adult
Biochemistry - methods
Blood Specimen Collection - instrumentation
Blood Specimen Collection - methods
Centrifugation
Female
Gels
Humans
Male
Middle Aged
Original Papers
Reference Values
Specimen Handling - instrumentation
Specimen Handling - methods
Temperature
Time Factors
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Summary:The collected and shipped blood samples are exposed to a various extra-analytical factors prior to analysis. The aim of the study was to determine the stability of analytes in serum gel tubes and plain tubes exposed to a range of storage temperatures and times after centrifugation. Fifteen healthy volunteers were recruited and venous blood was collected into four tubes, two with and two without gel separator. Analyzing the baseline samples in 30 min, all were stored at 4 degrees C or 24 degrees C for 6, 12, 18, 24, 30, 36, 48 and 72 hours and 1 week. Sixteen biochemical anaytes were measured on each sample. Variations remained under the desirable bias considered as clinically insignificant. On day three, most analytes remained stable including albumin, protein, creatinine, cholesterol, triglycerides, gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), alanine aminotransferase (ALT), creatine kinase (CK), lactate dehydrogenase (LD) regardless of tube types. Glucose concentration decreased markedly (P = 0.001) beginning from the first hours of storage in plain serum. The stability maximized for the analytes including glucose, total bilirubin, urea nitrogen (BUN), uric acid stored at 4 degrees C in gel tubes. Aspartate aminotransferase (AST) activity increased significantly (P = 0.002) up to 48-h, however bias was not significant clinically. High density lipoprotein (HDL) concentration was stable in gel tubes at 24 degrees C, in plain tubes at 4 degrees C stored up to 36-h. Serum gel or non-gel tubes might be used interchangeably for 11 analytes chilled or at 24 degrees C, whereas some restrictions must be applied for glucose, AST, BUN, HDL, and uric acid.
ISSN:1330-0962
1846-7482
1846-7482
DOI:10.11613/bm.2012.023