A comprehensive analysis of transfection-assisted delivery of iron oxide nanoparticles to dendritic cells

Abstract Polylysine (PL) has been used to facilitate dendritic cell (DC) uptake of super paramagnetic iron oxide (SPIO) nanoparticles for use in magnetic resonance imaging (MRI). In this work, we examined the effect of PL on cell toxicity and induction of cell maturation as manifested by the up-regu...

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Bibliographic Details
Published in:Nanomedicine 2013-11, Vol.9 (8), p.1235-1244
Main Authors: Toki, Shinji, PhD, Omary, Reed A., MD, Wilson, Kevin, MS, Gore, John C., PhD, Peebles, R. Stokes, MD, Pham, Wellington, PhD
Format: Article
Language:English
Subjects:
Animals
Bone Marrow Cells - cytology
Cell Death - drug effects
Cells, Cultured
Contrast Media - administration & dosage
Cytokines - analysis
Dendritic cells
Dendritic Cells - cytology
Dendritic Cells - drug effects
Dendritic Cells - ultrastructure
Drug Carriers - metabolism
Drug Carriers - toxicity
Ferric Compounds - administration & dosage
Humans
Internal Medicine
Iron oxide nanoparticles
Magnetic Resonance Imaging
Magnetite Nanoparticles - administration & dosage
Mice
Mice, Inbred C57BL
Nanotechnology
Polylysine
Polylysine - metabolism
Polylysine - toxicity
Transfection
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Summary:Abstract Polylysine (PL) has been used to facilitate dendritic cell (DC) uptake of super paramagnetic iron oxide (SPIO) nanoparticles for use in magnetic resonance imaging (MRI). In this work, we examined the effect of PL on cell toxicity and induction of cell maturation as manifested by the up-regulation of surface molecules. We found that PL became toxic to bone marrow-derived DCs (BMDCs) at the 10 μg/ml threshold. Incubation of BMDCs with 20 μg/ml of PL for 1 h resulted in approximately 90% cell death. However, addition of SPIO nanoparticles rescued DCs from PL-induced death as the combination of SPIO with PL did not cause cytotoxicity until the PL concentration was 1000 μg/ml. Prolonged exposure to PL induced BMDC maturation as noted by the expression of surface molecules such as MHC class II, CD40, CCR7 and CD86. However, the combination of SPIO and PL did not induce BMDC maturation at 1 h. However prolonged exposure to SPIO nanoparticles induced CD40 expression and protein expression of TNFα and KC. The data suggest that the use of PL to enhance the labeling of DCs with SPIO nanoparticles is a dedicated work. Appropriate calibration of the incubation time and concentrations of PL and SPIO nanoparticles is crucial to the development of MRI technology for noninvasive imaging of DCs in vivo. From the Clinical Editor The authors of this study present detailed data on toxicity and efficiency of polylysine-facilitated uptake of USPIO-s by dendritic cells for cell-specific MR imaging.
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2013.05.010