Comparative analysis of microRNA profiles between adult Ascaris lumbricoides and Ascaris suum

BACKGROUND: The parasitic nematodes Ascaris lumbricoides and A. suum are of great public health and economic significance, and the two taxa were proposed to represent a single species. miRNAs are known with functions of gene regulations at post-transcriptional level. RESULTS: We herein compared the...

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Bibliographic Details
Published in:BMC veterinary research 2014-04, Vol.10 (1), p.99-99, Article 99
Main Authors: Shao, Chang-Chun, Xu, Min-Jun, Alasaad, Samer, Song, Hui-Qun, Peng, Lifei, Tao, Jian-Ping, Zhu, Xing-Quan
Format: Article
Language:English
Subjects:
adults
Animals
Ascaris lumbricoides
Ascaris lumbricoides - genetics
Ascaris lumbricoides - metabolism
Ascaris suum
Ascaris suum - genetics
Ascaris suum - metabolism
bioinformatics
Biomarkers
Comparative analysis
correlation
Ethics
evolution
Female
Gene expression
Gene Expression Regulation
Genes
Genetic aspects
Genomes
high-throughput nucleotide sequencing
Hogs
microRNA
MicroRNAs - genetics
MicroRNAs - metabolism
NADH dehydrogenase
Parasites
prediction
proteoglycans
quantitative polymerase chain reaction
Real-Time Polymerase Chain Reaction
Ribosomal DNA
Science
Transcriptome
Veterinary medicine
vitellogenin
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Summary:BACKGROUND: The parasitic nematodes Ascaris lumbricoides and A. suum are of great public health and economic significance, and the two taxa were proposed to represent a single species. miRNAs are known with functions of gene regulations at post-transcriptional level. RESULTS: We herein compared the miRNA profiles of A. lumbricoides and A. suum female adults by Solexa deep sequencing combined with bioinformatics analysis and stem-loop real-time PCR. Using the A. suum genome as the reference genome, we obtained 171 and 494 miRNA candidates from A. lumbricoides and A. suum, respectively. Among which, 74 miRNAs were shared between the two taxa, 97 and 420 miRNAs were A. lumbricoides and A. suum specific. Target and function prediction revealed a significant set of targets which are related to ovarian message protein, vitellogenin and chondroitin proteoglycan of the two nematodes. Enrichment analysis revealed that the percentages of most predicted functions of the miRNA targets were similar, with some taxon specific or taxon enhanced functions, such as different target numbers, specific functions (NADH dehydrogenase and electron carrier functions), etc. CONCLUSIONS: This study characterized comparatively the miRNAs of adult A. lumbricoides and A. suum, and the findings provide additional evidence that A. lumbricoides and A. suum represent a single species. Due to the fast evolution nature of miRNAs and the different parasitic living conditions of humans and pigs, the phenomenon above might indicate a fast evolution of miRNAs of Ascaris in humans and pigs.
ISSN:1746-6148
1746-6148
DOI:10.1186/1746-6148-10-99