Atrial fibrosis in a chronic murine model of obstructive sleep apnea: mechanisms and prevention by mesenchymal stem cells

OSA increases atrial fibrillation (AF) risk and is associated with poor AF treatment outcomes. However, a causal association is not firmly established and the mechanisms involved are poorly understood. The aims of this work were to determine whether chronic obstructive sleep apnea (OSA) induces an a...

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Bibliographic Details
Published in:Respiratory research 2014-04, Vol.15 (1), p.54-54, Article 54
Main Authors: Ramos, Pablo, Rubies, Cira, Torres, Marta, Batlle, Montserrat, Farre, Ramon, Brugada, Josep, Montserrat, Josep M, Almendros, Isaac, Mont, Lluís
Format: Article
Language:English
Subjects:
Air flow
Analysis
Animals
Arrhythmia
Arítmia
Bone marrow
Cardiac arrhythmia
Cardiovascular disease
Cell culture
Cells, Cultured
Chronic Disease
Cèl·lules mare
Disease Models, Animal
Fibrosis
Heart Atria - pathology
Heart Atria - surgery
Heart diseases
Hypertension
Interleukins
Malalties del cor
Male
Mesenchymal Stem Cell Transplantation - methods
Rats
Rats, Inbred Lew
Rats, Sprague-Dawley
Rodents
Sleep apnea
Sleep apnea syndromes
Sleep Apnea, Obstructive - pathology
Sleep Apnea, Obstructive - prevention & control
Sleep Apnea, Obstructive - surgery
Sleep disorders
Stem cells
Studies
Síndromes d'apnea del son
Transplantation
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Summary:OSA increases atrial fibrillation (AF) risk and is associated with poor AF treatment outcomes. However, a causal association is not firmly established and the mechanisms involved are poorly understood. The aims of this work were to determine whether chronic obstructive sleep apnea (OSA) induces an atrial pro-arrhythmogenic substrate and to explore whether mesenchymal stem cells (MSC) are able to prevent it in a rat model of OSA. A custom-made setup was used to mimic recurrent OSA-like airway obstructions in rats. OSA-rats (n = 16) were subjected to 15-second obstructions, 60 apneas/hour, 6 hours/day during 21 consecutive days. Sham rats (n = 14) were placed in the setup but no obstructions were applied. In a second series of rats, MSC were administered to OSA-rats and saline to Sham-rats. Myocardial collagen deposit was evaluated in Picrosirius-red stained samples. mRNA expression of genes involved in collagen turnover, inflammation and oxidative stress were quantified by real time PCR. MMP-2 protein levels were quantified by Western Blot. A 43% greater interstitial collagen fraction was observed in the atria, but not in the ventricles, of OSA-rats compared to Sham-rats (Sham 8.32 ± 0.46% vs OSA 11.90 ± 0.59%, P 
ISSN:1465-993X
1465-9921
1465-993X
1465-9921
DOI:10.1186/1465-9921-15-54