Establishment and characterization of primary lung cancer cell lines from Chinese population

Aim： To establish and characterize primary lung cancer cell lines from Chinese population. Methods： Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium （for non-small cell lung carcinomas （NSCLC）） or HITES medium （for sma...

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Bibliographic Details
Published in:Acta pharmacologica Sinica 2011-03, Vol.32 (3), p.385-392
Main Authors: Zheng, Chao, Sun, Yi-hua, Ye, Xiao-lei, Chen, Hai-quan, Ji, Hong-bin
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Adenocarcinoma of Lung
Animals
Asian Continental Ancestry Group
Carcinoma, Non-Small-Cell Lung - genetics
Carcinoma, Non-Small-Cell Lung - metabolism
Carcinoma, Non-Small-Cell Lung - pathology
Carcinoma, Small Cell - genetics
Carcinoma, Small Cell - metabolism
Carcinoma, Small Cell - pathology
Cell Line, Tumor
ErbB Receptors - genetics
ErbB Receptors - metabolism
Humans
Lung cancer
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Mice
Mice, Nude
Mutation
Neoplasm Transplantation
Original
中国人口
原发性
基因突变
细胞生长动力学
细胞系
表皮生长因子受体
非小细胞肺癌
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Summary:Aim： To establish and characterize primary lung cancer cell lines from Chinese population. Methods： Lung cancer specimens or pleural effusions were collected from Chinese lung cancer patients and cultured in vitro with ACL4 medium （for non-small cell lung carcinomas （NSCLC）） or HITES medium （for small cell lung carcinomas （SCLC）） supplemented with 5% FBS. All cell lines were maintained in culture for more than 25 passages. Most of these cell lines were further analyzed for oncogenic mutations, karyotype, cell growth kinetics, and tumorigenicity in nude mice. Results： Eight primary cell lines from Chinese lung cancer patients were established and characterized, including seven NSCLC cell lines and one SCLC cell line. Five NSCLC cell lines were found to harbor epidermal growth factor receptor （EGFR） kinase domain mutations. Conclusion： These well-characterized primary lung cancer cell lines from Chinese population provide a unique platform for future stud- ies of the ethnic differences in lung cancer biology and drug response.
ISSN:1671-4083
1745-7254
DOI:10.1038/aps.2010.214