Contractile effect of tachykinins on rabbit small intestine

To study the role of the tachykinin receptors in spontaneous contractions of longitudinal and circular smooth muscle from rabbit small intestine and to determine the mechanism of action of Substance P (SP). Rabbit duodenum, jejunum and ileum segments were prepared. The spontaneous contractions of lo...

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Bibliographic Details
Published in:Acta pharmacologica Sinica 2011-04, Vol.32 (4), p.487-494
Main Authors: Valero, Marta Sofía, Fagundes, Diego Santos, Grasa, Laura, Arruebo, María Pilar, Plaza, Miguel Ángel, Murillo, María Divina
Format: Article
Language:English
Subjects:
Animals
Intestine, Small - drug effects
Intestine, Small - physiology
Male
Original
Rabbits
Tachykinins - pharmacology
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Summary:To study the role of the tachykinin receptors in spontaneous contractions of longitudinal and circular smooth muscle from rabbit small intestine and to determine the mechanism of action of Substance P (SP). Rabbit duodenum, jejunum and ileum segments were prepared. The spontaneous contractions of longitudinal and circular smooth muscle were recorded using a computer via an isometric force transducer. The specific agonists and antagonists of tachykinin receptors were added into the organ bath. The agonists of tachykinin NK1 receptor (SP and [Sar9] SP), NK2 receptor (NKA and (β-Ala8)-NKA), and NK3 receptor (NKB and Senktide) all induced contractions in the small intestine. The contractions were diminished by NK1 receptor antagonist L-733,060, NK2 receptor antagonist GR-94800, and NK3 receptor antagonist SB 218795. Contractions caused by SP were also reduced by atropine, verapamil, PKC inhibitor staurosporine, and PLC inhibitor U73122. Ttachykinin NK1, NK2, and NK3 receptors mediate the contractions of the smooth muscle in rabbit intestine. Furthermore, SP acts directly on smooth muscle cells through the tachykinin NK1 receptor.
ISSN:1671-4083
1745-7254
DOI:10.1038/aps.2010.227