A critical role for thymic stromal lymphopoietin in nickel-induced allergy in mice

Ni is the most frequent cause of contact allergy induced by metals. However, the underlying mechanism of this induction is unknown. Our previous research demonstrates that activation of dendritic cells (DCs) through p38MAPK/MKK6 is required for Ni-induced allergy in mice. In the current study, we in...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of immunology (1950) 2014-05, Vol.192 (9), p.4025-4031
Main Authors: Ashrin, Meinar Nur, Arakaki, Rieko, Yamada, Akiko, Kondo, Tomoyuki, Kurosawa, Mie, Kudo, Yasusei, Watanabe, Megumi, Ichikawa, Tetsuo, Hayashi, Yoshio, Ishimaru, Naozumi
Format: Article
Language:English
Subjects:
Allergy and Other Hypersensitivities
Animals
Blotting, Western
Cytokines - immunology
Cytokines - metabolism
Dendritic Cells - immunology
Dendritic Cells - metabolism
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Hypersensitivity - immunology
Hypersensitivity - metabolism
Immunoglobulins - immunology
Immunoglobulins - metabolism
Immunohistochemistry
Mice
Mice, Inbred C57BL
Nickel - immunology
Oligonucleotide Array Sequence Analysis
Real-Time Polymerase Chain Reaction
Receptors, Cytokine - immunology
Receptors, Cytokine - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Small Interfering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ni is the most frequent cause of contact allergy induced by metals. However, the underlying mechanism of this induction is unknown. Our previous research demonstrates that activation of dendritic cells (DCs) through p38MAPK/MKK6 is required for Ni-induced allergy in mice. In the current study, we investigated the cellular and molecular mechanisms underlying Ni-induced allergy using a mouse model that involves injecting Ni into the ear, with or without Freund's incomplete or complete adjuvants. Nickel had greater potential to cause allergic reactions compared with palladium and gold. Among the proteins expressed at higher levels in mice with Ni-induced allergy, we focused on thymic stromal lymphopoietin (TSLP), which is produced in abundance by keratinocytes. We detected increased expression of the TSLP receptor (TSLPR) in DCs from cervical lymph nodes of mice with Ni-induced allergy, suggesting that DCs in ear tissues were activated through TSLPR signaling induced by keratinocyte-derived TSLP. Furthermore, delayed-type hypersensitivity reactions in mice with Ni-induced allergy were decreased significantly by injection of a Tslp-short interfering RNA along with atelocollagen in the ear skin. These results suggest that Ni allergy may be triggered by a TSLP/TSLPR-mediated interaction between epithelial and immune cells.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.1300276