The Effect of miRNA-122 in Regulating Fat Deposition in a Cell Line Model

ABSTRACT Accumulating evidence supports the role of miR‐122 in fatty liver disease. We investigated miR‐122 expression in a steatotic hepatocyte model, the effect of miR‐122 over‐expression and inhibition in the pathogenesis. Human hepatic cell line L02 was induced with oleic acid to establish the s...

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Published in:Journal of cellular biochemistry 2014-05, Vol.115 (5), p.839-846
Main Authors: Nie, Yu-Qiang, Cao, Jie, Zhou, Yong-Jian, Liang, Xia, Du, Yan-Lei, Wan, Yu-Jui Yvonne, Li, Yu-Yuan
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Adipocytes - metabolism
Apoptosis - genetics
Cell Line
Complementary DNA
Fatty liver
Fatty Liver - genetics
Fatty Liver - metabolism
Fatty Liver - pathology
FATTY LIVER DISEASE
Fluorescence
Gene Expression Regulation
HEPATOCYTE
Hepatocytes
Hepatocytes - metabolism
Hepatocytes - pathology
Humans
Inhibitors
Intracellular
Lipid Metabolism - genetics
Lipids
Liver diseases
MicroRNAs - genetics
miR-122
miRNA
Nucleotide sequence
Oleic acid
Pathogenesis
PCR
STEATOSIS
Transfection
Triglycerides
Triglycerides - metabolism
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Summary:ABSTRACT Accumulating evidence supports the role of miR‐122 in fatty liver disease. We investigated miR‐122 expression in a steatotic hepatocyte model, the effect of miR‐122 over‐expression and inhibition in the pathogenesis. Human hepatic cell line L02 was induced with oleic acid to establish the steatotic hepatocyte model. Intracellular lipid content was observed with laser scanning confocal microscope (LSCM), and triglyceride content was determined with kits. Total RNA was extracted and reversely transcribed into cDNA. miR‐122 expression was measured using qRT‐PCR. Subsequently, miR‐122 mimic and miR‐122 inhibitor were transfected into steatotic hepatocytes to observe their effect on intracellular lipid content. The lipid fluorescence intensity and triglyceride content within the steatotic hepatocytes were significantly higher than those in normal control (860.01 ± 26.52 vs. 257.77 ± 29.69 and 3.47 ± 0.12 vs. 1.85 ± 0.02 at 24 h) (P 
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.24725