Effect of ampicillin, streptomycin, penicillin and tetracycline on metal resistant and non-resistant Staphylococcus aureus

There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains res...

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Published in:International journal of environmental research and public health 2014-03, Vol.11 (3), p.3233-3255
Main Authors: Chudobova, Dagmar, Dostalova, Simona, Blazkova, Iva, Michalek, Petr, Ruttkay-Nedecky, Branislav, Sklenar, Matej, Nejdl, Lukas, Kudr, Jiri, Gumulec, Jaromir, Tmejova, Katerina, Konecna, Marie, Vaculovicova, Marketa, Hynek, David, Masarik, Michal, Kynicky, Jindrich, Kizek, Rene, Adam, Vojtech
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotics
Cadmium - pharmacology
Drug resistance
Drug Resistance, Bacterial
Lead - pharmacology
Microbial Sensitivity Tests
Penicillin
Staphylococcus aureus
Staphylococcus aureus - drug effects
Studies
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Summary:There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health.
ISSN:1660-4601
1661-7827
1660-4601
DOI:10.3390/ijerph110303233