Botrytis cinerea early secretome

The extracellular proteome, or secretome, of phytopathogenic fungi is presumed to be a key element of their infection strategy. Especially interesting constituents of this set are those proteins secreted at the beginning of the infection, during the germination of conidia on the plant surfaces or wo...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2010-08, Vol.10 (16), p.3020-3034
Main Authors: Espino, José J, Gutiérrez-Sánchez, Gerardo, Brito, Nélida, Shah, Punit, Orlando, Ron, González, Celedonio
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Botrytis
Botrytis cinerea
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Fungal Proteins - chemistry
Fungal Proteins - classification
Fungal Proteins - isolation & purification
Fungal Proteins - secretion
Host-Pathogen Interactions
Microbiology
Miscellaneous
Peptide Mapping
Proteins
Proteome - analysis
Proteome - secretion
Secretome
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:The extracellular proteome, or secretome, of phytopathogenic fungi is presumed to be a key element of their infection strategy. Especially interesting constituents of this set are those proteins secreted at the beginning of the infection, during the germination of conidia on the plant surfaces or wounds, since they may play essential roles in the establishment of a successful infection. We have germinated Botrytis cinerea conidia in conditions that resemble the plant environment, a synthetic medium enriched with low molecular weight plant compounds, and we have collected the proteins secreted during the first 16 h by a double precipitation protocol. 2-D electrophoresis of the precipitated secretome showed a spot pattern similar for all conditions evaluated and for the control medium without plant extract. The proteins in 16 of these spots were identified by PMF and corresponded to 11 different polypeptides. Alternative determination of secretome composition by LC-MS/MS of tryptic fragments rendered a much larger number, 105 proteins, which included all previously identified by PMF. All proteins were functionally classified according to their putative function in the infection process. Key features of the early secretome include a large number of proteases, the abundance of proteins involved in the degradation of plant defensive barriers, and plenty of proteins with unknown function.
ISSN:1615-9853
1615-9861
1615-9861
DOI:10.1002/pmic.201000037