Natural History of Experimental Arterial Chronic Total Occlusions

Objectives We sought to perform the first systematic study of the natural history of chronic total arterial occlusions (CTOs) in an experimental model. Background Angioplasty of CTOs has low success rates. The structural and perfusion changes during CTO maturation, which may adversely affect angiopl...

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Published in:	Journal of the American College of Cardiology 2009-03, Vol.53 (13), p.1148-1158
Main Authors:	Jaffe, Ronen, MD, Leung, General, MSc, Munce, Nigel R., MSc, Thind, Amandeep S., BSc, Leong-Poi, Howard, MD, Anderson, Kevan J.T., BSc, Qi, Xiuling, PhD, MD, Trogadis, Judy, BSc, Nadler, Ariella, BA, Shiff, Davida, Saperia, Jamie, Lockwood, Julia, Jacobs, Chaim, Qiang, Beiping, MD, Teitelbaum, Aaron, MD, MSc, Dick, Alexander J., MD, Sparkes, John D., MSc, Butany, Jagdish, MD, Wright, Graham A., PhD, Strauss, Bradley H., MD, PhD
Format:	Article
Language:	eng
Subjects:	Angioplasty Animals Biological and medical sciences Blood Volume Cardiology Cardiology. Vascular system Cardiovascular Chronic Disease chronic total occlusions Collagen Disease Models, Animal Extracellular Matrix - pathology Femoral Artery Internal Medicine Magnetic Resonance Imaging Male Medical imaging Medical sciences Neovascularization, Pathologic NMR Nuclear magnetic resonance Rabbits Software Studies Success Thrombosis - pathology Thrombosis - physiopathology Tomography, X-Ray Computed - methods Veins & arteries
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Summary:	Objectives We sought to perform the first systematic study of the natural history of chronic total arterial occlusions (CTOs) in an experimental model. Background Angioplasty of CTOs has low success rates. The structural and perfusion changes during CTO maturation, which may adversely affect angioplasty outcome, have not been systematically studied. Methods Occlusions were created in 63 rabbit femoral arteries by thrombin injection. Histology, contrast-enhanced magnetic resonance imaging, relative blood volume (RBV) index, and micro-computed tomography imaging were analyzed at 2, 6, 12, and 18 to 24 weeks. Results Early changes were characterized by an acute inflammatory response and negative arterial remodeling, with >70% reduction of arterial cross-sectional area (CSA) from 2 to 6 weeks. Intraluminal neovascularization of the CTO occurred with a 2-fold increase in total (media + intima) microvessel CSA from 2 to 6 weeks (0.014 ± 0.002 mm2 to 0.023 ± 0.005 mm2 , p = 0.0008) and a 3-fold increase in RBV index (5.1 ± 1.9% to 16.9 ± 2.7%, p = 0.0008). However at later time periods, there were significant reductions in both RBV (3.5 ± 1.1%, p < 0.0001) and total microvessel CSA (0.017 ± 0.002 mm2 , p = 0.011). Micro-computed tomography imaging demonstrated a corkscrew-like recanalization channel at the proximal end at 6 weeks that regressed at later time points. These vascular changes were accompanied by a marked decrease in proteoglycans and accumulation of a collagen-enriched extracellular matrix, particularly at the entrance (“proximal fibrous cap”). Conclusions This study is the first to systematically analyze compositional changes occurring during CTO maturation, which may underlie angioplasty failure. Negative remodeling, regression of intraluminal channels, and CTO perfusion, together with the accumulation of dense collagen, may represent important targets for novel therapeutic interventions.
ISSN:	0735-1097 1558-3597