Physiological characterization of thermotolerant yeast for cellulosic ethanol production

The conversion of lignocellulose into fermentable sugars is considered a promising alternative for increasing ethanol production. Higher fermentation yield has been achieved through the process of simultaneous saccharification and fermentation (SSF). In this study, a comparison was performed between...

Full description

Saved in:
Bibliographic Details
Published in:Applied microbiology and biotechnology 2014-04, Vol.98 (8), p.3829-3840
Main Authors: Costa, Daniela A, de Souza, Carlos J. A, Costa, Patrícia S, Rodrigues, Marina Q. R. B, dos Santos, Ancély F, Lopes, Mariana R, Genier, Hugo L. A, Silveira, Wendel B, Fietto, Luciano G
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Alcohol
Alcohol, Denatured
Analysis
Bioenergy and Biofuels
Biological
Biomass
Biomedical and Life Sciences
Biotechnology
Brazil
Cellulose - metabolism
Comparative analysis
Drug Tolerance
Energy consumption
Enzymes
Ethanol
Ethanol - metabolism
Ethanol - toxicity
ethanol production
Ethyl alcohol
Fermentation
Glucose
heat tolerance
Industrial Microbiology
industry
Kluyveromyces - growth & development
Kluyveromyces - metabolism
Kluyveromyces marxianus
Life Sciences
Lignocellulose
Microbial Genetics and Genomics
Microbiology
Microorganisms
Physiological aspects
Physiology
saccharification
Saccharomyces cerevisiae
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Strain
Studies
Sugarcane
sugars
Yeast
Yeast fungi
yeasts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The conversion of lignocellulose into fermentable sugars is considered a promising alternative for increasing ethanol production. Higher fermentation yield has been achieved through the process of simultaneous saccharification and fermentation (SSF). In this study, a comparison was performed between the yeast species Saccharomyces cerevisiae and Kluyveromyces marxianus for their potential use in SSF process. Three strains of S. cerevisiae were evaluated: two are widely used in the Brazilian ethanol industry (CAT-1 and PE-2), and one has been isolated based on its capacity to grow and ferment at 42 °C (LBM-1). In addition, we used thermotolerant strains of K. marxianus. Two strains were obtained from biological collections, ATCC 8554 and CCT 4086, and one strain was isolated based on its fermentative capacity (UFV-3). SSF experiments revealed that S. cerevisiae industrial strains (CAT-1 and PE-2) have the potential to produce cellulosic ethanol once ethanol had presented yields similar to yields from thermotolerant strains. The industrial strains are more tolerant to ethanol and had already been adapted to industrial conditions. Moreover, the study shows that although the K. marxianus strains have fermentative capacities similar to strains of S. cerevisiae, they have low tolerance to ethanol. This characteristic is an important target for enhancing the performance of this yeast in ethanol production.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-014-5580-3