Investigating the feasibility of scale up and automation of human induced pluripotent stem cells cultured in aggregates in feeder free conditions

•First published protocol for scalable automation of hiPSC in feeder-free conditions.•Successful transfer of hiPSC between sites representative of research and manufacture.•Comparability between manual and automated expansion protocols for hiPSC. The transfer of a laboratory process into a manufactu...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biotechnology 2014-03, Vol.173 (100), p.53-58
Main Authors: Soares, Filipa A.C., Chandra, Amit, Thomas, Robert J., Pedersen, Roger A., Vallier, Ludovic, Williams, David J.
Format: Article
Language:English
Subjects:
Aggregates
Automated
Automation
Biomarkers - analysis
Biotechnology
Cell Aggregation
Cell Culture Techniques - instrumentation
Cell Differentiation
Cell Line
Clumps
Culture Media - chemistry
Equipment Design
Feeder free
Gene Expression
Human
Humans
Induced pluripotent stem cells
Induced Pluripotent Stem Cells - cytology
Manuals
Manufacturing
Microscopy, Fluorescence
Platforms
Robotics
Scale up
Short Communication
Stem cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•First published protocol for scalable automation of hiPSC in feeder-free conditions.•Successful transfer of hiPSC between sites representative of research and manufacture.•Comparability between manual and automated expansion protocols for hiPSC. The transfer of a laboratory process into a manufacturing facility is one of the most critical steps required for the large scale production of cell-based therapy products. This study describes the first published protocol for scalable automated expansion of human induced pluripotent stem cell lines growing in aggregates in feeder-free and chemically defined medium. Cells were successfully transferred between different sites representative of research and manufacturing settings; and passaged manually and using the CompacT SelecT automation platform. Modified protocols were developed for the automated system and the management of cells aggregates (clumps) was identified as the critical step. Cellular morphology, pluripotency gene expression and differentiation into the three germ layers have been used compare the outcomes of manual and automated processes.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2013.12.009