H460 non-small cell lung cancer stem-like holoclones yield tumors with increased vascularity

Abstract Cancer stem-like cells were isolated from several human tumor cell lines by limiting dilution assays and holoclone morphology, followed by assessment of self-renewal capacity, tumor growth, vascularity, and blood perfusion. H460 holoclone-derived tumors grew slower than parental H460 tumors...

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Bibliographic Details
Published in:Cancer letters 2014-04, Vol.346 (1), p.63-73
Main Authors: Manley, Eugene, Waxman, David J
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Cancer stem-like cell
Carcinoma, Non-Small-Cell Lung - genetics
Carcinoma, Non-Small-Cell Lung - pathology
Cell adhesion & migration
Cell culture
Cell Culture Techniques - methods
Cell growth
Cell Line, Tumor
Clone Cells - cytology
Colon
H460 holoclone
Hematology, Oncology and Palliative Medicine
Humans
Hypoxia
Immunohistochemistry
Lung cancer
Lung Neoplasms - genetics
Lung Neoplasms - pathology
Melanoma
Mice
Microarray
Neoplastic Stem Cells - metabolism
Neoplastic Stem Cells - pathology
Neovascularization, Pathologic - genetics
Neovascularization, Pathologic - pathology
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Prostate cancer
Small leucine-rich proteoglycan
Stem cells
Tumors
Xenograft Model Antitumor Assays
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Summary:Abstract Cancer stem-like cells were isolated from several human tumor cell lines by limiting dilution assays and holoclone morphology, followed by assessment of self-renewal capacity, tumor growth, vascularity, and blood perfusion. H460 holoclone-derived tumors grew slower than parental H460 tumors, but displayed significantly increased microvessel density and tumor blood perfusion. Microarray analysis identified 177 differentially regulated genes in the holoclone-derived tumors, of which 47 were associated with angiogenesis. The dysregulated genes include several small leucine-rich proteoglycans that may modulate angiogenesis and serve as novel therapeutic targets for inhibiting cancer stem cell-driven angiogenesis.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2013.12.012