Direct isolation of flavonoids from plants using ultra‐small anatase TiO₂ nanoparticles

Surface functionalization of nanoparticles has become an important tool for in vivo delivery of bioactive agents to their target sites. Here we describe the reverse strategy, nanoharvesting, in which nanoparticles are used as a tool to isolate bioactive compounds from living cells. Anatase TiO₂ nano...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 2014-02, Vol.77 (3), p.443-453
Main Authors: Kurepa, Jasmina, Nakabayashi, Ryo, Paunesku, Tatjana, Suzuki, Makoto, Saito, Kazuki, Woloschak, Gayle E, Smalle, Jan A
Format: Article
Language:English
Subjects:
anatase TiO2 nanoparticles
Anthocyanins - chemistry
Anthocyanins - isolation & purification
Arabidopsis - chemistry
Arabidopsis thaliana
bioactive properties
catechol
catechols
Catechols - chemistry
Catechols - isolation & purification
Cells
chemistry
flavonoids
Flavonoids - chemistry
Flavonoids - isolation & purification
Flowers & plants
Nanoparticles
Phosphorylation
plant tissues
Quercetin - chemistry
Quercetin - isolation & purification
solvents
stress
technical advance
Titanium - chemistry
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Summary:Surface functionalization of nanoparticles has become an important tool for in vivo delivery of bioactive agents to their target sites. Here we describe the reverse strategy, nanoharvesting, in which nanoparticles are used as a tool to isolate bioactive compounds from living cells. Anatase TiO₂ nanoparticles smaller than 20 nm form strong bonds with molecules bearing enediol and especially catechol groups. We show that these nanoparticles enter plant cells, conjugate enediol and catechol group‐rich flavonoids in situ, and exit plant cells as flavonoid‐nanoparticle conjugates. The source plant tissues remain viable after treatment. As predicted by the surface chemistry of anatase TiO₂ nanoparticles, quercetin‐based flavonoids were enriched amongst the nanoharvested flavonoid species. Nanoharvesting eliminates the use of organic solvents, allows spectral identification of the isolated compounds, and opens new avenues for use of nanomaterials for coupled isolation and testing of bioactive properties of plant‐synthesized compounds.
ISSN:0960-7412
1365-313X
DOI:10.1111/tpj.12361