Electrophysiology of human cardiac atrial and ventricular telocytes

Telocytes (TCs) with exceptionally long cellular processes of telopodes have been described in human epicardium to act as structural supporting cells in the heart. We examined myocardial chamber‐specific TCs identified in atrial and ventricular fibroblast culture using immunocytochemistry and studie...

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Bibliographic Details
Published in:Journal of cellular and molecular medicine 2014-02, Vol.18 (2), p.355-362
Main Authors: Sheng, Jingwei, Shim, Winston, Lu, Jun, Lim, Sze Yun, Ong, Boon Hean, Lim, Tien Siang, Liew, Reginald, Chua, Yeow Leng, Wong, Philip
Format: Article
Language:English
Subjects:
Antigens, CD34 - genetics
Antigens, CD34 - metabolism
Calcium conductance
Calcium ions
Cardiac arrhythmia
Cardiomyocytes
CD34 antigen
Cell culture
Cell Separation
Cells, Cultured
Electrophysiology
Epicardium
Fibroblasts
Fibroblasts - cytology
Fibroblasts - physiology
fibrosis
Gene Expression
Heart
Heart Atria - cytology
Heart Atria - metabolism
Heart Ventricles - cytology
Heart Ventricles - metabolism
Humans
Hydrogen sulfide
Hydrogen Sulfide - pharmacology
hydrogen sulphide
Immunocytochemistry
Immunoglobulins
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits - genetics
Large-Conductance Calcium-Activated Potassium Channel alpha Subunits - metabolism
Membrane Potentials - drug effects
Microscopy
Myocardium - cytology
Myocardium - metabolism
Original
Patch-Clamp Techniques
Potassium
potassium channel
Potassium channels (inwardly-rectifying)
Potassium channels (voltage-gated)
Potassium Channels, Inwardly Rectifying - genetics
Potassium Channels, Inwardly Rectifying - metabolism
Potassium conductance
Proto-Oncogene Proteins c-kit - genetics
Proto-Oncogene Proteins c-kit - metabolism
Receptor, Platelet-Derived Growth Factor beta - genetics
Receptor, Platelet-Derived Growth Factor beta - metabolism
Software
Stromal Cells - cytology
Stromal Cells - drug effects
Stromal Cells - physiology
Telocyte
Transforming growth factor
Transforming Growth Factor beta1 - pharmacology
Transforming growth factor-b1
Ventricle
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Description
Summary:Telocytes (TCs) with exceptionally long cellular processes of telopodes have been described in human epicardium to act as structural supporting cells in the heart. We examined myocardial chamber‐specific TCs identified in atrial and ventricular fibroblast culture using immunocytochemistry and studied their electrophysiological property by whole‐cell patch clamp. Atrial and ventricular TCs with extended telopodes and alternating podoms and podomers that expressed CD34, c‐Kit and PDGFR‐β were identified. These cells expressed large conductance Ca2+‐activated K+ current (BKCa) and inwardly rectifying K+ current (IKir), but not transient outward K+ current (Ito) and ATP‐sensitive potassium current (KATP). The active channels were functionally competent with demonstrated modulatory response to H2S and transforming growth factor (TGF)‐β1 whereby H2S significantly inhibited the stimulatory effect of TGF‐β1 on current density of both BKCa and IKir. Furthermore, H2S attenuated TGF‐β1‐stimulated KCa1.1/Kv1.1 (encode BKCa) and Kir2.1 (encode IKir) expression in TCs. Our results show that functionally competent K+ channels are present in human atrial and ventricular TCs and their modulation may have significant implications in myocardial physiopathology.
ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.12240