Adhesion Forces and Composition of Planktonic and Adhering Oral Microbiomes

The oral microbiome consists of a planktonic microbiome residing in saliva and an adhering microbiome (the biofilm adhering to oral hard and soft tissues). Here we hypothesized that possible differences in microbial composition of the planktonic and adhering oral microbiome on teeth can be related t...

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Bibliographic Details
Published in:Journal of dental research 2014-01, Vol.93 (1), p.84-88
Main Authors: Wessel, S.W., Chen, Y., Maitra, A., van den Heuvel, E.R., Slomp, A.M., Busscher, H.J., van der Mei, H.C.
Format: Article
Language:English
Subjects:
Adult
Animals
Atomic force microscopy
Bacteria
Bacterial Adhesion - physiology
Biofilms
Biomechanical Phenomena
Cattle
Coatings
Denaturing Gradient Gel Electrophoresis
Dental enamel
Dental Enamel - microbiology
Dental Pellicle - microbiology
Dental plaque
Dentistry
Enamel
Female
Gel electrophoresis
Humans
Hypotheses
Laboratories
Lactobacillus - isolation & purification
Lactobacillus - physiology
Lactobacillus acidophilus - isolation & purification
Lactobacillus acidophilus - physiology
Male
Microbiomes
Microbiota - physiology
Microscopy
Microscopy, Atomic Force
Middle Aged
Mouth - microbiology
Oral cavity
Research Reports
Saliva
Saliva - microbiology
Soft tissues
Species
Strains (organisms)
Streptococcus - isolation & purification
Streptococcus - physiology
Streptococcus mitis - isolation & purification
Streptococcus mitis - physiology
Streptococcus mutans - isolation & purification
Streptococcus mutans - physiology
Streptococcus oralis - isolation & purification
Streptococcus oralis - physiology
Streptococcus sanguis - isolation & purification
Streptococcus sanguis - physiology
Streptococcus sobrinus - isolation & purification
Streptococcus sobrinus - physiology
Studies
Tooth - microbiology
Young Adult
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Summary:The oral microbiome consists of a planktonic microbiome residing in saliva and an adhering microbiome (the biofilm adhering to oral hard and soft tissues). Here we hypothesized that possible differences in microbial composition of the planktonic and adhering oral microbiome on teeth can be related to the forces by which different bacterial species are attracted to the tooth surface. The relative presence of 7 oral bacterial species in saliva and biofilm collected from 10 healthy human volunteers was determined twice in each volunteer by denaturing-gradient-gel electrophoresis. Analysis of both microbiomes showed complete separation of the planktonic from the adhering oral microbiome. Next, adhesion forces of corresponding bacterial strains with saliva-coated enamel surfaces were measured by atomic force microscopy. Species that were found predominantly in the adhering microbiome had significantly higher adhesion forces to saliva-coated enamel (-0.60 to -1.05 nN) than did species mostly present in the planktonic microbiome (-0.40 to -0.55 nN). It is concluded that differences in composition of the planktonic and the adhering oral microbiome are due to small differences in the forces by which strains adhere to saliva-coated enamel, providing an important step in understanding site- and material-specific differences in the composition of biofilms in the oral cavity.
ISSN:0022-0345
1544-0591
1544-0591
DOI:10.1177/0022034513511822