Imaging of thyroid tumor angiogenesis with microbubbles targeted to vascular endothelial growth factor receptor type 2 in mice

To evaluate whether Contrast Enhanced Ultrasund (CEUS) with microbubbles (MBs) targeted to VEGFR-2 is able to characterize in vivo the VEGFR-2 expression in the tumor vasculature of a mouse model of thyroid cancer (Tg-TRK-T1). Animal protocol was approved by Institutional committee on Laboratory Ani...

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Bibliographic Details
Published in:BMC medical imaging 2013-09, Vol.13 (1), p.31-31, Article 31
Main Authors: Mancini, Marcello, Greco, Adelaide, Salvatore, Giuliana, Liuzzi, Raffaele, Di Maro, Gennaro, Vergara, Emilia, Chiappetta, Gennaro, Pasquinelli, Rosa, Brunetti, Arturo, Salvatore, Marco
Format: Article
Language:English
Subjects:
Animals
Biomarkers, Tumor - metabolism
Contrast Media - pharmacokinetics
Diagnosis
Diagnostic immunohistochemistry
Genetic engineering
Immunohistochemistry
Ionizing radiation
Kinases
Methods
Mice
Mice, Transgenic
Molecular Imaging - methods
Neovascularization, Pathologic - diagnostic imaging
Neovascularization, Pathologic - metabolism
Reproducibility of Results
Sensitivity and Specificity
Studies
Technical Advance
Thyroid cancer
Thyroid Neoplasms - diagnostic imaging
Thyroid Neoplasms - metabolism
Tumors
Ultrasonography - methods
Ultrasound imaging
Vascular Endothelial Growth Factor Receptor-2 - metabolism
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Summary:To evaluate whether Contrast Enhanced Ultrasund (CEUS) with microbubbles (MBs) targeted to VEGFR-2 is able to characterize in vivo the VEGFR-2 expression in the tumor vasculature of a mouse model of thyroid cancer (Tg-TRK-T1). Animal protocol was approved by Institutional committee on Laboratory Animal Care. Contrast-enhanced ultrasound imaging with MBs targeted with an anti-VEGFR-2 monoclonal antibody (UCAVEGFR-2) and isotype control antibody (UCAIgG) was performed in 7 mice with thyroid carcinoma, 5 mice with hyperplasia or benign thyroid nodules and 4 mice with normal thyroid. After ultrasonography, the tumor samples were harvested for histological examination and VEGFR-2 expression was tested by immunohistochemistry. Data were reported as median and range. Paired non parametric Wilcoxon's test and ANOVA of Kruskal-Wallis were used. The correlation between the contrast signal and the VEGFR-2 expression was assessed by the Spearman coefficient. The Video intensity difference (VID) caused by backscatter of the retained UCAVEGFR-2 was significantly higher in mice harboring thyroid tumors compared to mice with normal thyroids (P < 0.01) and to mice harboring benign nodules (P < 0.01). No statistically significant differences of VID were observed in the group of mice carrying benign nodules compared to mice with normal thyroids. Moreover in thyroid tumors VID of retained VEGFR-2-targeted UCA was significantly higher than that of control UCAIgG (P
ISSN:1471-2342
1471-2342
DOI:10.1186/1471-2342-13-31