The cohesin complex regulates immunoglobulin class switch recombination

Immunoglobulin (Ig) class switch recombination (CSR) is initiated by the transcription-coupled recruitment of activation-induced cytidine deaminase (AID) to switch regions and by the subsequent generation of double-stranded DNA breaks (DSBs). These DNA breaks are ultimately resolved through the nonh...

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Bibliographic Details
Published in:The Journal of experimental medicine 2013-11, Vol.210 (12), p.2495-2502
Main Authors: Thomas-Claudepierre, Anne-Sophie, Schiavo, Ebe, Heyer, Vincent, Fournier, Marjorie, Page, Adeline, Robert, Isabelle, Reina-San-Martin, Bernardo
Format: Article
Language:English
Subjects:
Animals
Brief Definitive Report
Cell Cycle Proteins - antagonists & inhibitors
Cell Cycle Proteins - genetics
Cell Cycle Proteins - metabolism
Cell Line
Chondroitin Sulfate Proteoglycans - metabolism
Chromosomal Proteins, Non-Histone - antagonists & inhibitors
Chromosomal Proteins, Non-Histone - genetics
Chromosomal Proteins, Non-Histone - metabolism
Cohesins
Cytidine Deaminase - metabolism
DNA End-Joining Repair
Gene Knockdown Techniques
Immunoglobulin Class Switching
Mice
Recombination, Genetic
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Summary:Immunoglobulin (Ig) class switch recombination (CSR) is initiated by the transcription-coupled recruitment of activation-induced cytidine deaminase (AID) to switch regions and by the subsequent generation of double-stranded DNA breaks (DSBs). These DNA breaks are ultimately resolved through the nonhomologous end joining (NHEJ) pathway. We show that during CSR, AID associates with subunits of cohesin, a complex previously implicated in sister chromatid cohesion, DNA repair, and the formation of DNA loops between enhancers and promoters. Furthermore, we implicate the cohesin complex in the mechanism of CSR by showing that cohesin is dynamically recruited to the Sμ-Cμ region of the IgH locus during CSR and that knockdown of cohesin or its regulatory subunits results in impaired CSR and increased usage of microhomology-based end joining.
ISSN:0022-1007
1540-9538
DOI:10.1084/jem.20130166