Remarks on the validity of Myxobolus ampullicapsulatus and Myxobolus honghuensis (Myxozoa: Myxosporea) based on SSU rDNA sequences

In the present study, we isolated three populations of Myxobolus ampullicapsulatus from the gills of crucian carp, Carassius auratus auratus, two from Yongchuan, Chongqing area and one from Poyang Lake, Jiangxi area, China, sequenced their complete small subunit ribosome RNA gene, analyzed their gen...

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Bibliographic Details
Published in:Parasitology research (1987) 2013-11, Vol.112 (11), p.3817-3823
Main Authors: Zhao, Y. J, Li, N. N, Tang, F. H, Dong, J. L
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Carassius auratus
Carassius auratus auratus
Carassius auratus gibelio
Carps - parasitology
China
Cluster Analysis
DNA sequencing
DNA, Protozoan - chemistry
DNA, Protozoan - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
genes
Genes, rRNA
Genetic aspects
genetic distance
Genetic Variation
gills
Gills - parasitology
goldfish
Immunology
lakes
Medical Microbiology
Microbiology
Molecular Sequence Data
Myxobolus
Myxobolus - classification
Myxobolus - cytology
Myxobolus - genetics
Myxobolus - isolation & purification
Myxosporea
Myxozoa
Myxozoans
nucleotide sequences
Nucleotide sequencing
Original Paper
pharynx
Phylogeny
Physiological aspects
ribosomal DNA
RNA
RNA, Protozoan - genetics
RNA, Ribosomal, 18S - genetics
sequence analysis
Sequence Analysis, DNA
spores
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Summary:In the present study, we isolated three populations of Myxobolus ampullicapsulatus from the gills of crucian carp, Carassius auratus auratus, two from Yongchuan, Chongqing area and one from Poyang Lake, Jiangxi area, China, sequenced their complete small subunit ribosome RNA gene, analyzed their genetic distance and gene similarity, and explored their relationship based on Bayesian inference and maximum likelihood analyses of their small subunit ribosomal DNA. The results combined with their morphological characteristics suggest that M. ampullicapsulatus infecting the gills and pharynx of allogynogenetic gibel carp, Carassius auratus gibelio, should be Myxobolus honghuensis. This study highlights the importance of DNA sequence comparisons for distinguishing Myxobolus species and indicates that the intra-species identification for the two Myxobolus species mentioned in the present research should be less than ten variation sites. In morphology, M. honghuensis Liu et al. (2012) parasitic on the gills of C. auratus auratus (goldfish) was collected from Chongqing area, and its mature spore was 16.5–19.5 × 8.5–10.0 μm in size, polar capsule was 7.0–10.0 × 2.5–4.0 μm in size, and polar filament had 9–10 coils. M. honghuensis Liu et al. (2012) isolated from the pharynx of C. auratus gibelio was sampled in Hubei area, and its mature spore was 15.1–19.5 × 9.0–11.3 μm in size, polar capsule was 7.9–8.1 × 3.0–4.5 μm in size, and polar filament had 7–8 coils.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-013-3569-y