Synthesis and properties of Asante Calcium Red—A novel family of long excitation wavelength calcium indicators

Abstract Although many synthetic calcium indicators are available, a search for compounds with improved characteristics continues. Here, we describe the synthesis and properties of Asante Calcium Red-1 (ACR-1) and its low affinity derivative (ACR-1-LA) created by linking BAPTA to seminaphthofluoresc...

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Bibliographic Details
Published in:Cell calcium (Edinburgh) 2013-10, Vol.54 (4), p.320-333
Main Authors: Hyrc, Krzysztof L, Minta, Akwasi, Escamilla, P. Rogelio, Chan, Patrick P.L, Meshik, Xenia A, Goldberg, Mark P
Format: Article
Language:English
Subjects:
Advanced Basic Science
Aniline Compounds - chemical synthesis
Aniline Compounds - chemistry
Animals
Calcium - metabolism
Calibration
Confocal microscopy
Dissociation constant
Emission spectra
Excitation spectra
Fluorescence
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Green Fluorescent Proteins - metabolism
HEK293 Cells
Heterocyclic Compounds, 4 or More Rings - chemical synthesis
Heterocyclic Compounds, 4 or More Rings - chemistry
Humans
Hydrogen-Ion Concentration
Indicators and Reagents - chemical synthesis
Indicators and Reagents - chemistry
Intracellular free calcium concentration
Ions
Light
Magnesium ions
Mice
Microscopy, Confocal
Multiphoton microscopy
Neurons
Neurons - cytology
Neurons - metabolism
Ratio imaging
Rats
Rats, Sprague-Dawley
Spectrometry, Fluorescence
Zinc ions
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Summary:Abstract Although many synthetic calcium indicators are available, a search for compounds with improved characteristics continues. Here, we describe the synthesis and properties of Asante Calcium Red-1 (ACR-1) and its low affinity derivative (ACR-1-LA) created by linking BAPTA to seminaphthofluorescein. The indicators combine a visible light (450–540 nm) excitation with deep-red fluorescence (640 nm). Upon Ca2+ binding, the indicators raise their fluorescence with longer excitation wavelengths producing higher responses. Although the changes occur without any spectral shifts, it is possible to ratio Ca2+ -dependent (640 nm) and quasi-independent (530 nm) emission when using visible (
ISSN:0143-4160
1532-1991
DOI:10.1016/j.ceca.2013.08.001