Staphylococcus aureus resistance on titanium coated with multivalent PEGylated-peptides

Abstract Bacterial infections can have adverse effects on the efficacy, lifetime and safety of an implanted device and are the second most commonly attributed cause of orthopedic implant failure. We have previously shown the assembly of PEGylated titanium-binding peptides (TBPs) on Ti to obtain a ba...

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Bibliographic Details
Published in:Biomaterials 2010-12, Vol.31 (35), p.9285-9292
Main Authors: Khoo, Xiaojuan, O’Toole, George A, Nair, Shrikumar A, Snyder, Brian D, Kenan, Daniel J, Grinstaff, Mark W
Format: Article
Language:English
Subjects:
Advanced Basic Science
Bacteria
Biofilm
Biofilms - drug effects
Biomaterial
Coated Materials, Biocompatible - chemistry
Coated Materials, Biocompatible - pharmacology
Dentistry
Implant
Microscopy, Atomic Force
Peptide
Peptides - chemistry
Peptides - pharmacology
Polyethylene Glycols - chemistry
Staphylococcus aureus
Staphylococcus aureus - drug effects
Surface modification
Titanium - chemistry
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Summary:Abstract Bacterial infections can have adverse effects on the efficacy, lifetime and safety of an implanted device and are the second most commonly attributed cause of orthopedic implant failure. We have previously shown the assembly of PEGylated titanium-binding peptides (TBPs) on Ti to obtain a bacteriophobic surface coating that can effectively resist protein adsorption and Staphylococcus aureus ( S. aureus ) adhesion. In the present study, we examine the effect of multiple TBP repeats on coating performance in vitro. Mono, di, and tetravalent peptides were synthesized and assessed for binding affinity and serum stability. PEGylated analogs were prepared and evaluated for their effect on S. aureus attachment and biofilm formation. Coating performance improved with the number of TBP repeats, with the tetravalent coating, TBP4 – PEG , showing the best performance in all assays. In particular, TBP4 – PEG forms a serum-resistant surface coating capable of preventing S. aureus colonization and subsequent biofilm formation. These results further support the role that multivalency can play in the development of improved surface coatings with enhanced stabilities and efficacy for in vivo clinical use.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2010.08.031