An easy, simple inexpensive test for the specific detection of Pectobacterium carotovorum subsp. carotovorum based on sequence analysis of the pmrA gene

The species Pectobacterium carotovorum includes a diverse subspecies of bacteria that cause disease on a wide variety of plants. In Morocco, approximately 95% of the P. carotovorum isolates from potato plants with tuber soft rot are P. carotovorum subsp. carotovorum. However, identification of this...

Full description

Saved in:
Bibliographic Details
Published in:BMC microbiology 2013-07, Vol.13 (1), p.176-176, Article 176
Main Authors: Kettani-Halabi, Mohamed, Terta, Meriam, Amdan, Mohamed, El Fahime, El Mostafa, Bouteau, François, Ennaji, Moulay Mustapha
Format: Article
Language:English
Subjects:
Bacteria, Phytopathogenic
Bacterial Proteins - genetics
Bacterial Typing Techniques - economics
Bacterial Typing Techniques - methods
Bacteriology
Biodiversity
Biological diversity
Contamination
Life Sciences
Microbiology and Parasitology
Molecular Sequence Data
Pectobacterium
Pectobacterium carotovorum - classification
Pectobacterium carotovorum - genetics
Pectobacterium carotovorum - isolation & purification
Phylogeny
Phytopathology and phytopharmacy
Plant Diseases - microbiology
Plant-pathogen relationships
Potatoes
Sequence Analysis, DNA - economics
Sequence Analysis, DNA - methods
Solanum tuberosum - microbiology
Species Specificity
Systematics, Phylogenetics and taxonomy
Vegetal Biology
Virulence (Microbiology)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The species Pectobacterium carotovorum includes a diverse subspecies of bacteria that cause disease on a wide variety of plants. In Morocco, approximately 95% of the P. carotovorum isolates from potato plants with tuber soft rot are P. carotovorum subsp. carotovorum. However, identification of this pathogen is not always related to visual disease symptoms. This is especially true when different pathogen cause similar diseases on potato, citing as an example, P. carotovorum, P. atrosepticum and P. wasabiae. Numerous conventional methods were used to characterize Pectobacterium spp., including biochemical assays, specific PCR-based tests, and construction of phylogenetic trees by using gene sequences. In this study, an alternative method is presented using a gene linked to pathogenicity, in order to allow accuracy at subspecies level. The pmrA gene (response regulator) has been used for identification and analysis of the relationships among twenty nine Pectobacterium carotovorum subsp. carotovorum and other Pectobacterium subspecies. Phylogenetic analyses of pmrA sequences compared to ERIC-PCR and 16S rDNA sequencing, demonstrated that there is considerable genetic diversity in P. carotovorum subsp. carotovorum strains, which can be divided into two distinct groups within the same clade. pmrA sequence analysis is likely to be a reliable tool to identify the subspecies Pectobacterium carotovorum subsp. carotovorum and estimate their genetic diversity.
ISSN:1471-2180
1471-2180
DOI:10.1186/1471-2180-13-176