Long-term multimodal imaging of tumor draining sentinel lymph nodes using mesoporous silica-based nanoprobes

Abstract The imaging of sentinel lymph nodes (SLNs), the first defense against primary tumor metastasis, has been considered as an important strategy for noninvasive tracking tumor metastasis in clinics. In this study, we report the development and application of mesoporous silica-based triple-modal...

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Bibliographic Details
Published in:Biomaterials 2012-06, Vol.33 (17), p.4370-4378
Main Authors: Huang, Xinglu, Zhang, Fan, Lee, Seulki, Swierczewska, Magdalena, Kiesewetter, Dale O, Lang, Lixin, Zhang, Guofeng, Zhu, Lei, Gao, Haokao, Choi, Hak Soo, Niu, Gang, Chen, Xiaoyuan
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Cell Line
Cell Line, Tumor
Dentistry
Disease Models, Animal
Female
Humans
Imaging, Three-Dimensional - methods
Lymph Nodes - diagnostic imaging
Lymph Nodes - pathology
Lymphatic Metastasis - diagnostic imaging
Macrophages - metabolism
Macrophages - pathology
Magnetic Resonance Imaging
Mesoporous silica nanoparticles
Mice
Mice, Inbred BALB C
Molecular Probes
Multimodality imaging
Nanoparticles - ultrastructure
Near-infrared fluorescence imaging
Neoplasms - diagnosis
Neoplasms - diagnostic imaging
Optics and Photonics
Porosity
Positron emission tomography
Sentinel Lymph Node Biopsy
Silicon Dioxide
Time Factors
Tissue Distribution
Tumor metastasis
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Summary:Abstract The imaging of sentinel lymph nodes (SLNs), the first defense against primary tumor metastasis, has been considered as an important strategy for noninvasive tracking tumor metastasis in clinics. In this study, we report the development and application of mesoporous silica-based triple-modal nanoprobes that integrate multiple functional moieties to facilitate near-infrared optical, magnetic resonance (MR) and positron emission tomography (PET) imaging. After embedding near-infrared dye ZW800, the nanoprobe was labeled with T1 contrast agent Gd3+ and radionuclide64 Cu through chelating reactions. High stability and long intracellular retention time of the nanoprobes was confirmed by in vitro characterization, which facilitate long-term in vivo imaging. Longitudinal multimodal imaging was subsequently achieved to visualize tumor draining SLNs up to 3 weeks in a 4T1 tumor metastatic model. Obvious differences in uptake rate, amount of particles, and contrast between metastatic and contra-lateral sentinel lymph nodes were observed. These findings provide very helpful guidance for the design of robust multifunctional nanomaterials in SLNs' mapping and tumor metastasis diagnosis.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2012.02.060