ATAF1 transcription factor directly regulates abscisic acid biosynthetic gene NCED3 in Arabidopsis thaliana

ATAF1, an Arabidopsis thaliana NAC transcription factor, plays important roles in plant adaptation to environmental stress and development. To search for ATAF1 target genes, we used protein binding microarrays and chromatin-immunoprecipitation (ChIP). This identified T[A,C,G]CGT[A,G] and TT[A,C,G]CG...

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Bibliographic Details
Published in:FEBS open bio 2013-01, Vol.3 (1), p.321-327
Main Authors: Jensen, Michael Krogh, Lindemose, Søren, Masi, Federico de, Reimer, Julia J., Nielsen, Michael, Perera, Venura, Workman, Chris T., Turck, Franziska, Grant, Murray R., Mundy, John, Petersen, Morten, Skriver, Karen
Format: Article
Language:English
Subjects:
Abscisic acid
Abscisic acid biosynthesis
Arabidopsis
Arabidopsis thaliana
Binding sites
Biosynthesis
Chromatin
Deoxyribonucleic acid
DNA
DNA microarrays
DNA-binding
Environmental stress
Experiments
Genes
Immunoprecipitation
Kinases
Menopause
NAC transcription factor
Protein arrays
Proteins
Regulation
Software
Transcription factors
Yeast
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Summary:ATAF1, an Arabidopsis thaliana NAC transcription factor, plays important roles in plant adaptation to environmental stress and development. To search for ATAF1 target genes, we used protein binding microarrays and chromatin-immunoprecipitation (ChIP). This identified T[A,C,G]CGT[A,G] and TT[A,C,G]CGT as ATAF1 consensus binding sequences. Co-expression analysis across publicly available microarray experiments identified 25 genes co-expressed with ATAF1. The promoter regions of ATAF1 co-expressors were significantly enriched for ATAF1 binding sites, and TTGCGTA was identified in the promoter of the key abscisic acid (ABA) phytohormone biosynthetic gene NCED3. ChIP-qPCR and expression analysis showed that ATAF1 binding to the NCED3 promoter correlated with increased NCED3 expression and ABA hormone levels. These results indicate that ATAF1 regulates ABA biosynthesis. •Consensus DNA-binding sites of the Arabidopsis transcription factor ATAF1 were identified.•The ATAF1 co-expression cluster is enriched for ATAF1 consensus binding sites•ABA biosynthetic gene NCED3 is co-expressed with ATAF1.•Plants over-expressing ATAF1 have increased ABA hormone levels.•ATAF1 binds in vivo to the promoter of NCED3 to activate expression.
ISSN:2211-5463
2211-5463
DOI:10.1016/j.fob.2013.07.006