Reduced expression of SK3 and IK1 channel proteins in the cavernous tissue of diabetic rats

The small （SK3） and intermediate （IK 1） conductance calcium-activated potassium channels could have key roles in the endothelium-dependent hyperpolarization factor pathway, which is believed to contribute to normal penile erection function. We aimed to investigate the expression of SK3 and IK1 in di...

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Bibliographic Details
Published in:Asian journal of andrology 2010-07, Vol.12 (4), p.599-604
Main Authors: Zhu, Jin-Hai, Jia, Rui-Peng, Xu, Lu-Wei, Wu, Jian-Ping, Wang, Zi-Zheng, Wang, Shu-Kui, Bo, Cheng-Jia
Format: Article
Language:English
Subjects:
Animals
Diabetes Mellitus, Experimental - metabolism
Diabetes Mellitus, Experimental - physiopathology
Intermediate-Conductance Calcium-Activated Potassium Channels - biosynthesis
Intermediate-Conductance Calcium-Activated Potassium Channels - genetics
Male
mRNA
Original
Penile Erection
Rats
Rats, Sprague-Dawley
RNA, Messenger - metabolism
Small-Conductance Calcium-Activated Potassium Channels - biosynthesis
Small-Conductance Calcium-Activated Potassium Channels - genetics
体组织
勃起功能障碍
糖尿病大鼠
逆转录聚合酶链反应
通道蛋白
阴茎勃起功能
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Summary:The small （SK3） and intermediate （IK 1） conductance calcium-activated potassium channels could have key roles in the endothelium-dependent hyperpolarization factor pathway, which is believed to contribute to normal penile erection function. We aimed to investigate the expression of SK3 and IK1 in diabetic rodents. The experimental diabetes model was induced in 8-week-old male Sprague-Dawley rats （250-300 g） by a single administration of streptozotocin. Both the diabetes mellitus group （DM group, n = 20） and the control group （NDM group, n = 10） were injected with a low dose of apomorphine to allow for the measurement and comparison of the corresponding penile erections. The mRNA and protein expression levels of SK3 and IK1 were measured by reverse transcription polymerase chain reaction and western blot, respectively. Erectile function was significantly decreased in the DM group compared with control group （P 〈 0.05）. The mRNA and protein expression levels of SK3 and IK1 were reduced in the cavernous tissue of diabetic rats compared with the control group （P 〈 0.05）. Diabetes inhibits mRNA and protein expression of both SK3 and IK1 in the cavernous tissue of diabetic rats. This could play a key role in the development of erectile dysfunction in diabetic rats.
ISSN:1008-682X
1745-7262
DOI:10.1038/aja.2009.97