Privileged Incorporation of Selenium as Selenocysteine in Lactobacillus reuteri Proteins Demonstrated by Selenium-specific Imaging and Proteomics

An analytical approach was developed to study the incorporation of selenium (Se), an important trace element involved in the protection of cells from oxidative stress, into the well-known probiotic Lactobacillus reuteri Lb2 BM-DSM 16143. The analyses revealed that about half of the internalized Se w...

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Bibliographic Details
Published in:Molecular & cellular proteomics 2013-08, Vol.12 (8), p.2196-2204
Main Authors: Galano, Eugenio, Mangiapane, Erika, Bianga, Juliusz, Palmese, Angelo, Pessione, Enrica, Szpunar, Joanna, Lobinski, Ryszard, Amoresano, Angela
Format: Article
Language:English
Subjects:
Bacterial Proteins - metabolism
Electrophoresis, Polyacrylamide Gel
Lactobacillus reuteri
Lactobacillus reuteri - growth & development
Lactobacillus reuteri - metabolism
Mass Spectrometry - methods
Proteomics
Selenium - metabolism
Selenocysteine - metabolism
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Summary:An analytical approach was developed to study the incorporation of selenium (Se), an important trace element involved in the protection of cells from oxidative stress, into the well-known probiotic Lactobacillus reuteri Lb2 BM-DSM 16143. The analyses revealed that about half of the internalized Se was covalently incorporated into soluble proteins. Se-enriched proteins were detected in 2D gels by laser ablation inductively coupled plasma mass spectrometry imaging (LA-ICP MSI) and identified by capillary HPLC with the parallel ICP MS (78Se) and electrospray Orbitrap MS/MS detection. On the basis of the identification of 10 richest in selenium proteins, it was demonstrated that selenium was incorporated by the strain exclusively as selenocysteine. Also, the exact location of selenocysteine within the primary sequence was determined. This finding is in a striking contrast to another common nutraceutical, Se-enriched yeast, which incorporates Se principally as selenomethionine.
ISSN:1535-9476
1535-9484
DOI:10.1074/mcp.M113.027607