Biodegradation of microcystin-RR by Bacillus flexus isolated from a Saudi freshwater lake

A bacterium capable of degrading microcystin-RR (MC-RR) was isolated from a Saudi eutrophic lake which was previously reported to have microcystin-producing cyanobacteria. Based on the analysis of the 16S rRNA gene sequence, the isolated strain SSZ01, most likely belong to the genus Bacillus with a...

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Bibliographic Details
Published in:Saudi journal of biological sciences 2012-10, Vol.19 (4), p.435-440
Main Author: Alamri, Saad A.
Format: Article
Language:English
Subjects:
16S rRNA gene
Bacillus
Bacillus flexus
bacteria
Biodegradation
Cyanobacteria
ecosystems
eutrophication
freshwater
genes
lakes
Microbiology
Microcystins
mlrA gene
nucleotide sequences
organic compounds
Original
ribosomal RNA
sequence homology
toxins
Water
المياه
علم الأحياء الدقيقة
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Summary:A bacterium capable of degrading microcystin-RR (MC-RR) was isolated from a Saudi eutrophic lake which was previously reported to have microcystin-producing cyanobacteria. Based on the analysis of the 16S rRNA gene sequence, the isolated strain SSZ01, most likely belong to the genus Bacillus with a highest sequence similarity (99%) with Bacillus flexus strain EMGA5. It was found that B. flexus strain SSZ01, possesses an mlrA gene encoding the most important enzyme for MC degradation. This strain was capable of degrading MC-RR, at a concentration of 10mgl−1, in batch experiments under environmentally relevant conditions. The degradation of MC-RR was completely removed within 4d. The degradation of MC-RR by this strain occurred in a rich medium nutrient broth (NB), indicating that this could likely occur along with other organic compounds found in the environment. Therefore, the coexistence of such bacteria with MCs in the same environment can contribute to the self-purification of the ecosystem from such potent toxins. This is the first study to report that B. flexus can degrade MCs.
ISSN:1319-562X
2213-7106
DOI:10.1016/j.sjbs.2012.06.006