Arabidopsis BPM Proteins Function as Substrate Adaptors to a CULLIN3-Based E3 Ligase to Affect Fatty Acid Metabolism in Plants

Regulation of transcriptional processes is a critical mechanism that enables efficient coordination of the synthesis of required proteins in response to environmental and cellular changes. Transcription factors require accurate activity regulation because they play a critical role as key mediators a...

Full description

Saved in:
Bibliographic Details
Published in:The Plant cell 2013-06, Vol.25 (6), p.2253-2264
Main Authors: Chen, Liyuan, Lee, Joo Hyun, Weber, Henriette, Tohge, Takayuki, Witt, Sandra, Roje, Sanja, Fernie, Alisdair R., Hellmann, Hanjo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antibodies
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Carrier Proteins - genetics
Carrier Proteins - metabolism
Chromatin Immunoprecipitation
Cycloheximide - pharmacology
Fatty acids
Fatty Acids - metabolism
Gene expression regulation
Gene Expression Regulation, Plant - drug effects
Genes
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Lipid metabolism
Mathematics
Microscopy, Confocal
Molecular Sequence Data
Nucleotidyltransferases - genetics
Nucleotidyltransferases - metabolism
Plant cells
Plants
Plants, Genetically Modified
Proteasome Endopeptidase Complex - metabolism
Protein Binding
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Seeds - genetics
Seeds - metabolism
Sequence Homology, Amino Acid
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Two-Hybrid System Techniques
Ubiquitin-Protein Ligases - genetics
Ubiquitin-Protein Ligases - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Regulation of transcriptional processes is a critical mechanism that enables efficient coordination of the synthesis of required proteins in response to environmental and cellular changes. Transcription factors require accurate activity regulation because they play a critical role as key mediators assuring specific expression of target genes. In this work, we show that CULLIN3-based E3 ligases have the potential to interact with a broad range of ETHYLENE RESPONSE FACTOR (ERF)/APETALA2 (AP2) transcription factors, mediated by MATH-BTB/POZ (for Meprin and TRAF [tumor necrosis factor receptor associated factor] homolog)-Broad complex, Tramtrack, Bric-a-brac/Pox virus and Zinc finger) proteins. The assembly with an E3 ligase causes degradation of their substrates via the 26S proteasome, as demonstrated for the WRINKLED1 ERF/AP2 protein. Furthermore, loss of MATH-BTB/POZ proteins widely affects plant development and causes altered fatty acid contents in mutant seeds. Overall, this work demonstrates a link between fatty acid metabolism and E3 ligase activities in plants and establishes CUL3-based E3 ligases as key regulators in transcriptional processes that involve ERF/AP2 family members.
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.112.107292