Genetic characterization of antimicrobial resistance of Shigella flexneri 1c isolates from patients in Egypt and Pakistan

Shigella flexneri serotype 1c emerged as a critical isolate from children in Egypt and Pakistan. The pattern of antimicrobial susceptibility (AMS) and resistance genes of this serotype have yet to be characterized. Sixty nine S. flexneri 1c isolates isolates were identified from both Egypt (n-46) an...

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Bibliographic Details
Published in:Annals of clinical microbiology and antimicrobials 2013-05, Vol.12 (1), p.9-9
Main Authors: Ahmed, Salwa F, Klena, John, Husain, Tupur, Monestersky, Jesse, Naguib, Amel, Wasfy, Momtaz O
Format: Article
Language:English
Subjects:
Adolescent
Ampicillin
Ampicillin - pharmacology
Anti-Bacterial Agents - pharmacology
Antibiotics
Antimicrobial agents
Bacterial Proteins - genetics
Bacterial Typing Techniques
beta-Lactamases - genetics
Carrier Proteins - genetics
Child
Child, Preschool
Disease susceptibility
Disk Diffusion Antimicrobial Tests
DNA sequencing
Drug resistance
Drug resistance in microorganisms
Drug Resistance, Multiple, Bacterial
Dysentery, Bacillary - diagnosis
Egypt
Genes
Genes, Bacterial
Genetic aspects
Humans
Microbiology
Nucleotide sequencing
Pakistan
Shigella flexneri
Shigella flexneri - classification
Shigella flexneri - drug effects
Shigella flexneri - genetics
Shigella flexneri - isolation & purification
Short Report
Trimethoprim - pharmacology
Young Adult
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Summary:Shigella flexneri serotype 1c emerged as a critical isolate from children in Egypt and Pakistan. The pattern of antimicrobial susceptibility (AMS) and resistance genes of this serotype have yet to be characterized. Sixty nine S. flexneri 1c isolates isolates were identified from both Egypt (n-46) and Pakistan (n = 23) and tested for AMS by disk diffusion method and minimal inhibitory concentrations were also determined. Isolates were genotyped by pulsed field gel electrophoresis (PFGE) and five relevant resistance genes (bla(TEM), bla(SHV), bla(OXA), sulI and sulII) were detected by polymerase chain reaction (PCR) and confirmed by DNA sequencing. High resistance was observed in all isolates for ampicillin (AM >96%); trimethoprim-sulphamethoxazole and tetracycline (>88%). Most AM-resistant isolates from Egypt (70%) harbored bla(TEM) resistance, while 52% of isolates from Pakistan expressed bla(OXA). All isolates were closely related by PFGE, irrespective of source or time of collection. The sulII gene was present in 100% of isolates from pediatric cases in Egypt, 65% of Pakistan isolates, and 53% of isolates from older Egyptian patients. While different Shigella serotypes gathered in specific genotypic groups, 1c serotype isolates formed multiple clusters. Although AMS was considerably high to most commonly used drugs, genetic determinants were variable between countries over time. The data stress the need for a more careful selection of antibiotics in the treatment of shigellosis.
ISSN:1476-0711
1476-0711
DOI:10.1186/1476-0711-12-9