Low-level laser therapy (LLLT) reduces oxidative stress in primary cortical neurons in vitro

Low‐level laser (light) therapy (LLLT) involves absorption of photons being in the mitochondria of cells leading to improvement in electron transport, increased mitochondrial membrane potential (MMP), and greater ATP production. Low levels of reactive oxygen species (ROS) are produced by LLLT in nor...

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Bibliographic Details
Published in:Journal of biophotonics 2013-10, Vol.6 (10), p.829-838
Main Authors: Huang, Ying-Ying, Nagata, Kazuya, Tedford, Clark E., McCarthy, Thomas, Hamblin, Michael R.
Format: Article
Language:English
Subjects:
Animals
Cell Survival - radiation effects
Cerebral Cortex - cytology
cobalt chloride
cultured cortical neurons
Cytoplasm - metabolism
Cytoplasm - radiation effects
Female
hydrogen peroxide
low-level laser therapy
Low-Level Light Therapy
Membrane Potential, Mitochondrial - radiation effects
Mice
Mitochondria - metabolism
Mitochondria - radiation effects
Neurons - cytology
Neurons - metabolism
Neurons - radiation effects
oxidative stress
Oxidative Stress - radiation effects
Pregnancy
reactive oxygen species
Superoxides - metabolism
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Summary:Low‐level laser (light) therapy (LLLT) involves absorption of photons being in the mitochondria of cells leading to improvement in electron transport, increased mitochondrial membrane potential (MMP), and greater ATP production. Low levels of reactive oxygen species (ROS) are produced by LLLT in normal cells that are beneficial. We exposed primary cultured murine cortical neurons to oxidative stressors: hydrogen peroxide, cobalt chloride and rotenone in the presence or absence of LLLT (3 J/cm2, CW, 810 nm wavelength laser, 20 mW/cm2). Cell viability was determined by PrestoblueTM assay. ROS in mitochondria was detected using Mito‐sox, while ROS in cytoplasm was detected with CellRoxTM. MMP was measured with tetramethylrhodamine. In normal neurons LLLT elevated MMP and increased ROS. In oxidatively‐stressed cells LLLT increased MMP but reduced high ROS levels and protected cultured cortical neurons from death. Although LLLT increases ROS in normal neurons, it reduces ROS in oxidatively‐stressed neurons. In both cases MMP is increased. These data may explain how LLLT can reduce clinical oxidative stress in various lesions while increasing ROS in cells in vitro. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201200157