Nicotine-induced cessation of embryonic development is reversed by γ-tocotrienol in mice

This study aimed to evaluate the adverse effects of various doses of nicotine and protective effects of different concentrations of gamma-tocotrienol (gamma-TCT) on in vitro embryonic development and lipid peroxidation in mice. A) Effects of various doses of nicotine on in vitro embryonic developmen...

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Bibliographic Details
Published in:Medical science monitor. Basic research 2013-03, Vol.19, p.87-92
Main Authors: Kamsani, Yuhaniza Shafinie, Rajikin, Mohd Hamim, Mohamed Nor Khan, Nor-Ashikin, Abdul Satar, Nuraliza, Chatterjee, Amar
Format: Article
Language:English
Subjects:
Animal Studies
Animals
Blastocyst - pathology
Chromans - pharmacology
Disease Models, Animal
Dose-Response Relationship, Drug
Embryo Culture Techniques - methods
Embryonic Development - drug effects
Female
Lipid Peroxidation
Malondialdehyde - blood
Mice
Nicotine - pharmacology
Thiobarbituric Acid Reactive Substances - analysis
Time Factors
Vitamin E - analogs & derivatives
Vitamin E - pharmacology
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Summary:This study aimed to evaluate the adverse effects of various doses of nicotine and protective effects of different concentrations of gamma-tocotrienol (gamma-TCT) on in vitro embryonic development and lipid peroxidation in mice. A) Effects of various doses of nicotine on in vitro embryonic development: Female mice were treated with 1.0, 3.0, or 5.0 mg/kg/day nicotine for 7 consecutive days. Animals were superovulated, cohabited overnight, and sacrificed. Embryos were cultured in vitro. Plasma was assayed. B) Effects of concomitant treatment of nicotine concurrently with various doses of gamma-TCT on in vitro embryonic development: Female mice were treated with nicotine (5.0 mg/kg/day), gavaged gamma-TCT of 30, 60, or 90 mg/kg/day or nicotine concurrently with gamma-TCT of 3 different doses for 7 consecutive days. Animals were superovulated, cohabited overnight, and sacrificed. Embryos were cultured and plasma was assayed. A) Effects of various doses of nicotine on in vitro embryonic development: Number of hatched blastocysts decreased in 1.0 and 3.0 mg/kg/day nicotine groups. Nicotine at 5.0 mg/kg/day stopped embryo development at morula. MDA concentrations increased following all nicotine doses. B) Effects of concomitant treatment of nicotine concurrently with various doses of gamma-TCT on in vitro embryonic development: Embryo development was completed in all groups. MDA concentration increased only in the group treated with nicotine concurrently with 30 mg/kg/day gamma-TCT. Nicotine impairs in vitro embryo development and increases MDA in plasma. The deleterious impact of nicotine on embryo development is reversed by supplementing gamma-TCT concurrently with nicotine.
ISSN:2325-4416
2325-4394
2325-4416
DOI:10.12659/MSMBR.883822