Analysis of 4‑Hydroxy-1-(3-pyridyl)-1-butanone (HPB)-Releasing DNA Adducts in Human Exfoliated Oral Mucosa Cells by Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry

Quantitation of DNA adducts could provide critical information on the relationship between exposure to tobacco smoke and cancer risk in smokers. In this study, we developed a robust and sensitive liquid chromatography–tandem mass spectrometry method for the analysis of 4-hydroxy-1-(3-pyridyl)-1-buta...

Full description

Saved in:
Bibliographic Details
Published in:Chemical research in toxicology 2013-01, Vol.26 (1), p.37-45
Main Authors: Stepanov, Irina, Muzic, John, Le, Chap T, Sebero, Erin, Villalta, Peter, Ma, Bin, Jensen, Joni, Hatsukami, Dorothy, Hecht, Stephen S
Format: Article
Language:English
Subjects:
Biomarkers - urine
Butanones - chemistry
Butanones - toxicity
Chromatography, High Pressure Liquid
DNA - chemistry
DNA - metabolism
DNA Adducts - analysis
DNA Adducts - isolation & purification
Humans
Mouth Mucosa - chemistry
Mouth Mucosa - drug effects
Mouth Mucosa - metabolism
Mouthwashes - chemistry
Nitrosamines - chemistry
Nitrosamines - metabolism
Pyridines - chemistry
Pyridines - toxicity
Smoking
Solid Phase Extraction
Spectrometry, Mass, Electrospray Ionization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Quantitation of DNA adducts could provide critical information on the relationship between exposure to tobacco smoke and cancer risk in smokers. In this study, we developed a robust and sensitive liquid chromatography–tandem mass spectrometry method for the analysis of 4-hydroxy-1-(3-pyridyl)-1-butanone (HPB)-releasing DNA adducts in human oral cells, a noninvasive source of DNA for biomarker studies. Isolated DNA undergoes acid hydrolysis, after which samples are purified by solid-phase extraction and analyzed by LC-ESI-MS/MS. The developed method was applied to the analysis of samples obtained via collection with a commercial mouthwash from 30 smokers and 15 nonsmokers. In smokers, the levels of HPB-releasing DNA adducts averaged 12.0 pmol HPB/mg DNA (detected in 20 out of 28 samples with quantifiable DNA yield), and in nonsmokers, the levels of adducts averaged 0.23 pmol/mg DNA (detected in 3 out of 15 samples). For the 30 smoking subjects, matching buccal brushings were also analyzed, and HPB-releasing DNA adducts were detected in 24 out of 27 samples with quantifiable DNA yield, averaging 44.7 pmol HPB/mg DNA. The levels of adducts in buccal brushings correlated with those in mouthwash samples of smokers (R = 0.73, p < 0.0001). Potentially, the method can be applied in studies of individual susceptibility to tobacco-induced cancers in humans.
ISSN:0893-228X
1520-5010
1520-5010
DOI:10.1021/tx300282k