Regulation of secondary wall synthesis and cell death by NAC transcription factors in the monocot Brachypodium distachyon

In several dicotyledonous species, NAC transcription factors act as master switches capable of turning on programmes of secondary cell-wall synthesis and cell death. This work used an oestradiol-inducible system to overexpress the NAC transcription factor BdSWN5 in the monocot model Brachypodium dis...

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Bibliographic Details
Published in:Journal of experimental botany 2013-03, Vol.64 (5), p.1333-1343
Main Authors: Valdivia, Elene R, Herrera, María Teresa, Gianzo, Cristina, Fidalgo, Javier, Revilla, Gloria, Zarra, Ignacio, Sampedro, Javier
Format: Article
Language:English
Subjects:
apoptosis
Base Sequence
binding sites
Binding Sites - genetics
Biological and medical sciences
Brachypodium - cytology
Brachypodium - genetics
Brachypodium - metabolism
Brachypodium distachyon
Cell Death
Cell Wall - genetics
Cell Wall - metabolism
Cell walls
cellulose synthase
Electrophoretic Mobility Shift Assay
Fundamental and applied biological sciences. Psychology
gene expression regulation
Gene Expression Regulation, Plant
Genes
Genes, Plant - genetics
Molecular Sequence Data
Nicotiana - cytology
Nicotiana - genetics
Organ Specificity - genetics
Phylogeny
Plant cells
Plant Leaves - cytology
Plant Leaves - genetics
Plant physiology and development
Plant Proteins - genetics
Plant Proteins - metabolism
Plants
Plants, Genetically Modified
Promoter Regions, Genetic - genetics
Protein Binding - genetics
proteinases
RESEARCH PAPER
Rice
roots
Seedlings
shoots
tracheary elements
Transactivation
Transcription factors
Transcription Factors - genetics
Transcription Factors - metabolism
Transcriptional Activation
Xylem
Xylem - genetics
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Summary:In several dicotyledonous species, NAC transcription factors act as master switches capable of turning on programmes of secondary cell-wall synthesis and cell death. This work used an oestradiol-inducible system to overexpress the NAC transcription factor BdSWN5 in the monocot model Brachypodium distachyon. This resulted in ectopic secondary cell-wall formation in both roots and shoots. Some of the genes upregulated in the process were a secondary cell-wall cellulose synthase (BdCESA4), a xylem-specific protease (BdXCP1) and an orthologue of AtMYB46 (BdMYB1). While activation of BdMYB1 may not be direct, this study showed that BdSWN5 is capable of transactivating the BdXCP1 promoter through two conserved binding sites. In the course of Brachypodium development, the BdXCP1 promoter was observed to be active in all types of differentiating tracheary elements. Together, these results suggest that Brachypodium SWNs can act as switches that turn on secondary cell-wall synthesis and programmed cell death.
ISSN:0022-0957
1460-2431
DOI:10.1093/jxb/ers394