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Cloning and expression of feline colony stimulating factor receptor (CSF-1R) and analysis of the species specificity of stimulation by colony stimulating factor-1 (CSF-1) and interleukin-34 (IL-34)
► Full-length feline CSF-1R has been cloned and expressed in a stable cell line. ► The biological activity of human, mouse and porcine CSF-1 has been evaluated. ► Human and mouse IL-34 are also biologically active on the feline CSF-1R. ► The potential therapeutic applications of CSF-1 is discussed....
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Published in: | Cytokine (Philadelphia, Pa.) Pa.), 2013-02, Vol.61 (2), p.630-638 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | ► Full-length feline CSF-1R has been cloned and expressed in a stable cell line. ► The biological activity of human, mouse and porcine CSF-1 has been evaluated. ► Human and mouse IL-34 are also biologically active on the feline CSF-1R. ► The potential therapeutic applications of CSF-1 is discussed.
Colony stimulating factor (CSF-1) and its receptor, CSF-1R, have been previously well studied in humans and rodents to dissect the role they play in development of cells of the mononuclear phagocyte system. A second ligand for the CSF-1R, IL-34 has been described in several species. In this study, we have cloned and expressed the feline CSF-1R and examined the responsiveness to CSF-1 and IL-34 from a range of species. The results indicate that pig and human CSF-1 and human IL-34 are equally effective in cats, where both mouse CSF-1 and IL-34 are significantly less active. Recombinant human CSF-1 can be used to generate populations of feline bone marrow and monocyte derived macrophages that can be used to further dissect macrophage-specific gene expression in this species, and to compare it to data derived from mouse, human and pig. These results set the scene for therapeutic use of CSF-1 and IL-34 in cats. |
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ISSN: | 1043-4666 1096-0023 |
DOI: | 10.1016/j.cyto.2012.11.014 |