Pre-treatment with Bifidobacterium breve UCC2003 modulates Citrobacter rodentium-induced colonic inflammation and organ specificity

Citrobacter rodentium, which colonizes the gut mucosa via formation of attaching and effacing (A/E) lesions, causes transmissible colonic hyperplasia. The aim of this study was to evaluate whether prophylactic treatment with Bifidobacterium breve UCC2003 can improve the outcome of C. rodentium infec...

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Bibliographic Details
Published in:Microbiology (Society for General Microbiology) 2012-11, Vol.158 (Pt 11), p.2826-2834
Main Authors: COLLINS, James W, AKIN, Ali R, KOSTA, Artemis, NING ZHANG, TANGNEY, Mark, FRANCIS, Kevin P, FRANKEL, Gad
Format: Article
Language:English
Subjects:
Animals
Bacteriology
Bifidobacterium - physiology
Biological and medical sciences
Citrobacter rodentium - immunology
Citrobacter rodentium - physiology
Colon - immunology
Colon - microbiology
Colon - pathology
Colonic Diseases - immunology
Colonic Diseases - microbiology
Colonic Diseases - pathology
Colonic Diseases - prevention & control
Enterobacteriaceae Infections - immunology
Enterobacteriaceae Infections - microbiology
Enterobacteriaceae Infections - pathology
Enterobacteriaceae Infections - prevention & control
Female
Fundamental and applied biological sciences. Psychology
Mice
Mice, Inbred C57BL
Microbial Pathogenicity
Microbiology
Miscellaneous
Organ Specificity
Probiotics - administration & dosage
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Summary:Citrobacter rodentium, which colonizes the gut mucosa via formation of attaching and effacing (A/E) lesions, causes transmissible colonic hyperplasia. The aim of this study was to evaluate whether prophylactic treatment with Bifidobacterium breve UCC2003 can improve the outcome of C. rodentium infection. Six-week-old albino C57BL/6 mice were pre-treated for 3 days with B. breve, challenged with bioluminescent C. rodentium and administered B. breve or PBS-C for 8 days post-infection; control mice were either administered B. breve and mock-infected with PBS, or mock-treated with PBS-C and mock-infected with PBS. C. rodentium colonization was monitored by bacterial enumeration from faeces and by a combination of both 2D bioluminescence imaging (BLI) and composite 3D diffuse light imaging tomography with µCT imaging (DLIT-µCT). At day 8 post-infection, colons were removed and assessed for crypt hyperplasia, histology by light microscopy, bacterial colonization by immunofluorescence, and A/E lesion formation by electron microscopy. Prophylactic administration of B. breve did not prevent C. rodentium colonization or A/E lesion formation. However, this treatment did alter C. rodentium distribution within the large intestine and significantly reduced colonic crypt hyperplasia at the peak of bacterial infection. These results show that B. breve could not competitively exclude C. rodentium, but reduced pathogen-induced colonic inflammation.
ISSN:1350-0872
1465-2080
DOI:10.1099/mic.0.060830-0