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The T cell receptor repertoires of regulatory and conventional T cells specific for the same foreign antigen are distinct1
The relationship between the TCR repertoires of natural regulatory T (nT reg ) and conventional T (T conv ) cells capable of responding to the same antigenic epitope is unknown. Here, we used TCRβ-chain transgenic mice to generate polyclonal nT reg and T conv cell populations specific for a foreign...
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| Published in: | The Journal of immunology (1950) 2012-08, Vol.189 (7), p.3566-3574 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Online Access: | Get full text |
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| Summary: | The relationship between the TCR repertoires of natural regulatory T (nT
reg
) and conventional T (T
conv
) cells capable of responding to the same antigenic epitope is unknown. Here, we used TCRβ-chain transgenic mice to generate polyclonal nT
reg
and T
conv
cell populations specific for a foreign antigen. CD4
+
T cells from immunized 3.L2β
+/−
TCRα
+/−
Foxp3
EGFP
mice were re-stimulated in culture to yield nT
reg
cells (EGFP
+
) and T
conv
cells (EGFP
−
) defined by their antigenic reactivity. Relative to T
conv
cells, nT
reg
cell expansion was delayed, although a higher proportion of viable nT
reg
cells had divided after 72 hours. Spectratype analysis revealed that both the nT
reg
and T
conv
cell responses were different and characterized by skewed distributions of CDR3 lengths. CDR3 sequences from nT
reg
cells displayed a divergent pattern of Jα usage, minimal CDR3 overlap (3.4%), and less diversity than CDR3 sequences derived from T
conv
cells. These data indicate that foreign antigen-specific nT
reg
and T
conv
cells are clonally distinct, and that foreign antigen-specific nT
reg
cells populations are constrained by a limited TCR repertoire. |
|---|---|
| ISSN: | 0022-1767 1550-6606 |
| DOI: | 10.4049/jimmunol.1102646 |