Amino-Terminated Generation 2 Poly(amidoamine) Dendrimer as a Potential Broad-Spectrum, Nonresistance-Inducing Antibacterial Agent

The treatment of septicemia caused by antibiotic-resistant bacteria is a great challenge in the clinic. Because traditional antibiotics inevitably induce bacterial resistance, which is responsible for many treatment failures, there is an urgent need to develop novel antibiotic drugs. Amino-terminate...

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Bibliographic Details
Published in:The AAPS journal 2013-01, Vol.15 (1), p.132-142
Main Authors: Xue, Xiaoyan, Chen, Xiaoqing, Mao, Xinggang, Hou, Zheng, Zhou, Ying, Bai, Hui, Meng, Jingru, Da, Fei, Sang, Guojun, Wang, Yukun, Luo, Xiaoxing
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Biochemistry
Biomedical and Life Sciences
Biomedicine
Biotechnology
Dendrimers - pharmacology
Drug Resistance, Bacterial
Escherichia coli
Female
Humans
Male
Mice
Mice, Inbred BALB C
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Pharmacology/Toxicology
Pharmacy
Polyamines - pharmacology
Research Article
Staphylococcus aureus
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Summary:The treatment of septicemia caused by antibiotic-resistant bacteria is a great challenge in the clinic. Because traditional antibiotics inevitably induce bacterial resistance, which is responsible for many treatment failures, there is an urgent need to develop novel antibiotic drugs. Amino-terminated Poly(amidoamine) dendrimers (PAMAM-NH 2 ) are reported to have antibacterial activities. However, previous studies focused on high generations of PAMAM-NH 2 , which have been found to exhibit high toxicities. The present study aimed to clarify whether low generations of PAMAM-NH 2 could be used as novel antibacterial agents. We found that generation 2 (G2.0) PAMAM-NH 2 showed significant antibacterial effects against antibiotic-sensitive and antibiotic-resistant strains but exhibited little toxicity to human gastric epithelial cells and did not induce antibiotic resistance in bacteria. Scanning and transmission electron microscopy analyses suggested that G2.0 PAMAM-NH 2 might inhibit the growth of bacteria by destroying their cell membranes. The administration of G2.0 PAMAM-NH 2 dose-dependently improved the animal survival rate of mice infected with extended-spectrum beta lactamase-producing Escherichia coli (ESBL-EC) and of animals infected with a combination of ESBL-EC and methicillin-resistant Staphylococcus aureus . A treatment regimen of 10 mg/kg of G2.0 PAMAM-NH 2 starting 12 h before inoculation followed by 10 mg/kg at 0.5 h after inoculation rescued 100% of singly infected mice and 60% of multiply infected mice. The protective effects were associated with the reduction of the bacterial titers in the blood and with the morphological amelioration of infected tissues. These findings demonstrate that the G2.0 PAMAM-NH 2 is a potential broad-spectrum and nonresistance-inducing antibiotic agent with relatively low toxicity.
ISSN:1550-7416
1550-7416
DOI:10.1208/s12248-012-9416-8