Modulation of hepatitis C virus RNA abundance and virus release by dispersion of processing bodies and enrichment of stress granules

Abstract Components of cytoplasmic processing bodies (P-bodies) and stress granules can be subverted during viral infections to modulate viral gene expression. Because hepatitis C virus (HCV) RNA abundance is regulated by P-body components such as microRNA miR-122, Argonaute 2 and RNA helicase RCK/p...

Full description

Saved in:
Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2013-01, Vol.435 (2), p.472-484
Main Authors: Pager, Cara T, Schütz, Sylvia, Abraham, Teresa M, Luo, Guangxiang, Sarnow, Peter
Format: Article
Language:English
Subjects:
Abundance
Argonaute 2 protein
Cell Line, Tumor
Core protein
Cytoplasm
Cytoplasmic Granules - metabolism
Data processing
Gene expression
Gene Expression Regulation, Viral
Granules
Hepacivirus - genetics
Hepacivirus - metabolism
Hepacivirus - pathogenicity
Hepacivirus - physiology
Hepatitis C virus
Hepatocytes - virology
Humans
Immunofluorescence
Infection
Infectious Disease
Lipid droplets
Lipids
miRNA
Packaging
Processing bodies
RNA helicase
RNA viruses
RNA, Viral - genetics
RNA, Viral - metabolism
Stress
Stress granules
Viral Proteins - genetics
Viral Proteins - metabolism
Viral RNA assembly
Virions
Virus Assembly
Virus Release - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Components of cytoplasmic processing bodies (P-bodies) and stress granules can be subverted during viral infections to modulate viral gene expression. Because hepatitis C virus (HCV) RNA abundance is regulated by P-body components such as microRNA miR-122, Argonaute 2 and RNA helicase RCK/p54, we examined whether HCV infection modulates P-bodies and stress granules during viral infection. It was discovered that HCV infection decreased the number of P-bodies, but induced the formation of stress granules. Immunofluorescence studies revealed that a number of P-body and stress granule proteins co-localized with viral core protein at lipid droplets, the sites for viral RNA packaging. Depletion of selected P-body proteins decreased overall HCV RNA and virion abundance. Depletion of stress granule proteins also decreased overall HCV RNA abundance, but surprisingly enhanced the accumulation of infectious, extracellular virus. These data argue that HCV subverts P-body and stress granule components to aid in viral gene expression at particular sites in the cytoplasm.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2012.10.027