Detection of Quorum Sensing Signal Molecules in Edwardsiella ictaluri Ei-151

Edwardsiella ictaluri is a Gram-negative pathogenic bacterium in the family Enterobacteriaceae that causes enteric septicemia of catfish, which has become a significant problem in the aquaculture of striped catfish (Pangasianodon hypophthalmus) in Vietnam. In this study, a bacterium designated as Ei...

Full description

Saved in:
Bibliographic Details
Published in:Indian journal of microbiology 2012-12, Vol.52 (4), p.581-586
Main Authors: Yang, Qian, Han, Yin, Tinh, Nguyen Thi Ngoc, Hien, Nguyen Thi, Bossier, Peter
Format: Article
Language:English
Subjects:
Agrobacterium radiobacter
Agrobacterium tumefaciens
Aquaculture
Bacteria
Biomedical and Life Sciences
Biosensors
catfish
Communication
Edwardsiella ictaluri
Enterobacteriaceae
Gram-positive bacteria
homoserine
Life Sciences
Medical Microbiology
Microbiology
Original
Original Article
Pangasianodon hypophthalmus
quorum sensing
ribosomal DNA
Sepsis
septicemia
Spleen
Virulence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Edwardsiella ictaluri is a Gram-negative pathogenic bacterium in the family Enterobacteriaceae that causes enteric septicemia of catfish, which has become a significant problem in the aquaculture of striped catfish (Pangasianodon hypophthalmus) in Vietnam. In this study, a bacterium designated as Ei-151 was isolated from diseased striped catfish and proved to be virulent. Based on 16S rDNA sequencing and phenotypic tests, the pathogenic bacterium was identified as Edw. ictaluri. The presence of quorum sensing signal molecules in Edw. ictaluri Ei-151 was detected with different biosensor strains. The results showed that Ei-151 produced at least three kinds of acylated homoserine lactone (AHL) signal molecules as detected with the biosensor Agrobacterium tumefaciens KYC55, and the AHLs fingerprint was similar to that of Edw. tarda. During its entire growth, the levels of AHLs and autoinducer-2 produced by Ei-151 peaked at the stationary phase (OD₆₀₀ 1.8), which suggested that both of them may function at the stationary phase. No Cholerae autoinducer-1-like activity (including Edw. ictaluri LMG7860ᵀ) was detected.
ISSN:0046-8991
0973-7715
DOI:10.1007/s12088-012-0312-9