Gene dosage effect of WEE1 on growth and morphogenesis from arabidopsis hypocotyl explants

• Background and Aims How plant cell-cycle genes interface with development is unclear. Preliminary evidence from our laboratory suggested that over-expression of the cell cycle checkpoint gene, WEE1, repressed growth and development. Here the hypothesis is tested that the level of WEE1 has a dosage...

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Published in:	Annals of botany 2012-12, Vol.110 (8), p.1631-1639
Main Authors:	Spadafora, Natasha, Perrotta, Lara, Nieuwland, Jeroen, Albani, Diego, Bitonti, M. Beatrice, Herbert, Robert J., Doonan, John H., Marchbank, Angela M., Siciliano, Ilario, Grønlund, Anne Lentz, Francis, Dennis, Rogers, Hilary J.
Format:	Article
Language:	eng
Subjects:	Adventitious roots Arabidopsis Arabidopsis - cytology Arabidopsis - drug effects Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Cell Count Cell cycle Cell Cycle - genetics Cell growth Cell Size Developmental biology Gene Dosage Gene Expression Gene Expression Regulation, Plant Genotypes Hypocotyl - cytology Hypocotyl - drug effects Hypocotyl - genetics Hypocotyl - growth & development Hypocotyls Kinetin - pharmacology Meristem - cytology Meristem - drug effects Meristem - genetics Meristem - growth & development Morphogenesis Mutagenesis, Insertional Naphthols - pharmacology Original Phenotype Plant Epidermis - cytology Plant Epidermis - genetics Plant Epidermis - growth & development Plant Growth Regulators - pharmacology Plant Roots - cytology Plant Roots - drug effects Plant Roots - genetics Plant Roots - growth & development Plants Plants, Genetically Modified Protein-Serine-Threonine Kinases - genetics Protein-Serine-Threonine Kinases - metabolism Root growth Seedlings Time Factors Tissue Culture Techniques
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Summary:	• Background and Aims How plant cell-cycle genes interface with development is unclear. Preliminary evidence from our laboratory suggested that over-expression of the cell cycle checkpoint gene, WEE1, repressed growth and development. Here the hypothesis is tested that the level of WEE1 has a dosage effect on growth and development in Arabidospis thaliana. To do this, a comparison was made of the development of gain-and loss-offunction WEE1 arabidopsis lines both in vivo and in vitro. • Methods Hypocotyl expiants from an over-expressing Arath; WEE1 line (WEE1oe), two T-DNA insertion lines (wee 1-1 and wee 1-4) and wild type (WT) were cultured on two-way combinations of kinetin and naphthyl acetic acid. Root growth and meristematic cell size were also examined. • Key Results Quantitative data indicated a repressive effect in WEE1oe and a significant increase in morphogenetic capacity in the two T-DNA insertion lines compared with WT. Compared with WT, WEE1oe seedlings exhibited a slower cell-doubling time in the root apical meristem and a shortened primary root, with fewer laterals, whereas there were no consistent differences in the insertion lines compared with WT. However, significantly fewer adventitious roots were recorded for WEE1oe and significantly more for the insertion mutant wee1-1. Compared with WT there was a significant increase in meristem cell size in WEE1oe for all three ground tissues but for wee1-1 only cortical cell size was reduced. • Conclusions There is a gene dosage effect of WEE1 on morphogenesis from hypocotyls both in vitro and in vivo.
ISSN:	0305-7364 1095-8290