Bacteriophages are synergistic with bacterial interference for the prevention of Pseudomonas aeruginosa biofilm formation on urinary catheters

Aims We hypothesized that pretreating urinary catheters with benign Escherichia coli HU2117 plus an antipseudomonal bacteriophage (ΦE2005‐A) would prevent Pseudomonas aeruginosa biofilm formation on catheters – a pivotal event in the pathogenesis of catheter‐associated urinary tract infection (CAUTI...

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Bibliographic Details
Published in:Journal of applied microbiology 2012-12, Vol.113 (6), p.1530-1539
Main Authors: Liao, K.S., Lehman, S.M., Tweardy, D.J., Donlan, R.M., Trautner, B.W.
Format: Article
Language:English
Subjects:
Antibiosis
Bacterial Adhesion
Bacterial infections
Bacteriology
bacteriophages
Biofilms
Biological and medical sciences
Catheter-Related Infections - prevention & control
Catheters
Escherichia coli
Escherichia coli - physiology
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
probiotics
pseudomonads
Pseudomonas aeruginosa
Pseudomonas aeruginosa - growth & development
Pseudomonas aeruginosa - virology
Pseudomonas Phages - physiology
Urinary Catheters - microbiology
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Summary:Aims We hypothesized that pretreating urinary catheters with benign Escherichia coli HU2117 plus an antipseudomonal bacteriophage (ΦE2005‐A) would prevent Pseudomonas aeruginosa biofilm formation on catheters – a pivotal event in the pathogenesis of catheter‐associated urinary tract infection (CAUTI). Methods and Results Silicone catheter segments were exposed to one of four pretreatments (sterile media; E. coli alone; phage alone; E. coli plus phage), inoculated with P. aeruginosa and then incubated up to 72 h in human urine before rinsing and sonicating to recover adherent bacteria. Pseudomonas aeruginosa adherence to catheters was almost 4 log10 units lower when pretreated with E. coli plus phage compared to no pretreatment (P 
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2012.05432.x