Characteristics of deslanoside-induced modulation on jejunal contractility

AIM： To characterize the dual effects of deslanoside on the contractility of jejunal smooth muscle. METHODS： Eight pairs of different low and high con- tractile states of isolated jejunal smooth muscle frag- ment （JSMF） were established. Contractile amplitude of JSMF in different low and high contra...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2012-11, Vol.18 (41), p.5889-5896
Main Authors: Chen, Da-Peng, Xiong, Yong-Jian, Tang, Ze-Yao, Yao, Qi-Ying, Ye, Dong-Mei, Liu, Sha-Sha, Lin, Yuan
Format: Article
Language:English
Subjects:
Adrenergic Antagonists - pharmacology
Animals
blot分析
Calcium - metabolism
Calcium Channel Blockers - pharmacology
Deslanoside - pharmacology
Enzyme Inhibitors - pharmacology
Gastrointestinal Motility - drug effects
In Vitro Techniques
Jejunum - drug effects
Jejunum - innervation
Jejunum - metabolism
Muscarinic Antagonists - pharmacology
Muscle Contraction - drug effects
Muscle, Smooth - drug effects
Muscle, Smooth - innervation
Muscle, Smooth - metabolism
Myosin Light Chains - metabolism
Nitric Oxide Synthase - antagonists & inhibitors
Nitric Oxide Synthase - metabolism
Original
Phosphorylation
Rats
Rats, Sprague-Dawley
Time Factors
低收缩
刺激作用
空肠
肌球蛋白轻链
肾上腺素能
蛋白磷酸化
调制特性
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Summary:AIM： To characterize the dual effects of deslanoside on the contractility of jejunal smooth muscle. METHODS： Eight pairs of different low and high con- tractile states of isolated jejunal smooth muscle frag- ment （JSMF） were established. Contractile amplitude of JSMF in different low and high contractile states was selected to determine the effects of deslanoside, and Western blotting analysis was performed to measure the effects of deslanoside on myosin phosphorylation of jejunal smooth muscle. RESULTS： Stimulatory effects on the contractility of JSMF were induced （45.3% ± 4.0% vs 87.0% ±7.8%, P 〈 0.01） by deslanoside in 8 low contractile states, and inhibitory effects were induced （180.6% ± 17.8% vs 109.9% ± 10.8%, P 〈 0.01） on the contractility of JSMF in 8 high contractile states. The effect of desla- noside on the phosphorylation of myosin light chain of JSMF in low （78.1% ± 4.1% vs 96.0% ± 8.1%, P 〈 0.01） and high contractile state （139.2% ± 8.5% vs 105.5 ± 7.34, P 〈 0.01） was also bidirectional. Bidirectional regulation （BR） was abolished in the presence of tetrodotoxin. Deslanoside did not affect jejunal con- tractility pretreated with the Ca2 channel blocker vera- pamil or in a Ca2-free assay condition. The stimulatory effect of deslanoside on JSMF in a low contractile state （low Ca2 induced） was abolished by atropine. The inhibitory effect of deslanoside on jejunal contractil- ity in a high contractile state （high Ca2 induced） was blocked by phentolamine, propranolol and L-NG-nitro- arginine, respectively. CONCLUSION： Deslanoside-induced BR is Ca2 dependent and is related to cholinergic and adrenergic systems when JSMF is in low or high contractile states,
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v18.i41.5889