Research Resource: RNA-Seq Reveals Unique Features of the Pancreatic β-Cell Transcriptome

The pancreatic β-cell is critical for the maintenance of glycemic control. Knowing the compendium of genes expressed in β-cells will further our understanding of this critical cell type and may allow the identification of future antidiabetes drug targets. Here, we report the use of next-generation s...

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Bibliographic Details
Published in:Molecular endocrinology (Baltimore, Md.) Md.), 2012-10, Vol.26 (10), p.1783-1792
Main Authors: Ku, Gregory M, Kim, Hail, Vaughn, Ian W, Hangauer, Matthew J, Myung Oh, Chang, German, Michael S, McManus, Michael T
Format: Article
Language:English
Subjects:
Animals
Female
Gene Expression Profiling
High-Throughput Nucleotide Sequencing
Insulin-Secreting Cells - metabolism
Male
Mice
Mice, Transgenic
Organ Specificity
Promoter Regions, Genetic
Research Resource
RNA Splicing
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Analysis, RNA
Transcriptome
Tryptophan Hydroxylase - genetics
Tryptophan Hydroxylase - metabolism
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Summary:The pancreatic β-cell is critical for the maintenance of glycemic control. Knowing the compendium of genes expressed in β-cells will further our understanding of this critical cell type and may allow the identification of future antidiabetes drug targets. Here, we report the use of next-generation sequencing to obtain nearly 1 billion reads from the polyadenylated RNA of islets and purified β-cells from mice. These data reveal novel examples of β-cell-specific splicing events, promoter usage, and over 1000 long intergenic noncoding RNA expressed in mouse β-cells. Many of these long intergenic noncoding RNA are β-cell specific, and we hypothesize that this large set of novel RNA may play important roles in β-cell function. Our data demonstrate unique features of the β-cell transcriptome.
ISSN:0888-8809
1944-9917
DOI:10.1210/me.2012-1176