Analysis of PFOA in dosed CD1 mice: Part 1. Methods development for the analysis of tissues and fluids from pregnant and lactating mice and their pups

The number of studies involving the analysis of perfluorooctanoic acid (PFOA) has increased recently because PFOA is routinely detected in human blood samples from around the world. Recent studies with mice have shown that dosing pregnant dams with PFOA during gestation gives rise to a dose-dependen...

Full description

Saved in:
Bibliographic Details
Published in:Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2009-06, Vol.27 (3-4), p.360-364
Main Authors: Reiner, Jessica L., Nakayama, Shoji F., Delinsky, Amy D., Stanko, Jason P., Fenton, Suzanne E., Lindstrom, Andrew B., Strynar, Mark J.
Format: Article
Language:English
Subjects:
Amniotic fluid
Amniotic Fluid - chemistry
Animals
Animals, Newborn
Blood Chemical Analysis - methods
Body Burden
Caprylates - administration & dosage
Caprylates - analysis
Caprylates - blood
Caprylates - pharmacokinetics
Caprylates - toxicity
Caprylates - urine
Dose-Response Relationship, Drug
Environmental Pollutants - administration & dosage
Environmental Pollutants - analysis
Environmental Pollutants - pharmacokinetics
Environmental Pollutants - toxicity
Female
Fluorocarbons - administration & dosage
Fluorocarbons - analysis
Fluorocarbons - blood
Fluorocarbons - pharmacokinetics
Fluorocarbons - toxicity
Fluorocarbons - urine
Lactation
Mammary tissue
Maternal Exposure
Mice
Mice, Inbred Strains
Milk
Milk - chemistry
PFOA
Pregnancy
Prenatal Exposure Delayed Effects - blood
Prenatal Exposure Delayed Effects - chemically induced
Prenatal Exposure Delayed Effects - metabolism
Prenatal Exposure Delayed Effects - urine
Reproductive
Serum
Time Factors
Tissue Distribution
Urinalysis
Urine
Whole pups
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The number of studies involving the analysis of perfluorooctanoic acid (PFOA) has increased recently because PFOA is routinely detected in human blood samples from around the world. Recent studies with mice have shown that dosing pregnant dams with PFOA during gestation gives rise to a dose-dependent mortality in the litters, a reduction in neonatal body weight for the surviving pups, and subsequent deficits in mammary gland development when compared to control animals. The actual body burdens of PFOA in dams and pups associated with these endpoints have not been determined, in part due to a lack of robust analytical methods for these matrices. The goal of the current study was to develop reliable methods with acceptable performance characteristics for the analysis of PFOA in several matrices relevant to pregnant mouse studies. Dam and pup serum, amniotic fluid, urine, milk, mammary tissue, and whole mouse pups were isolated for method development and analysis. The resulting method provided excellent accuracy (92.1–111%) and reproducibility (relative standard deviation 4.3–21%) making them very useful for future studies. These methods were then applied to dosed animal fluids and tissues in order to conduct a thorough evaluation of the pharmacokinetics in utero. Resulting tissue specific measurements of PFOA in serum, amniotic fluid, urine, milk, mammary tissue, and whole pup homogenate will be used to more completely describe the dose–response relationships for the most sensitive health outcomes and inform pharmacokinetic models that are being developed and evaluated.
ISSN:0890-6238
1873-1708
DOI:10.1016/j.reprotox.2008.10.006