Glucocorticoid Inhibition of Initiation of Transcription of the DNA Encoding rRNA (rDNA) in Lymphosarcoma P1798 Cells

Cell-free extracts of lymphosarcoma P1798 cell culture lines support faithful initiation upon the cloned mouse DNA encoding rRNA (rDNA) promoter, whereas extracts from cells treated for 16 hr with 0.1μ M dexamethasone cannot. Extracts from both sources transcribe the cloned 5S RNA gene in vitro and...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1984-02, Vol.81 (3), p.718-721
Main Authors: Cavanaugh, Alice H., Gokal, Preeti K., Lawther, Robert P., Thompson, E. Aubrey
Format: Article
Language:English
Subjects:
Animals
Cell extracts
Cell Line
Cell lines
Cricetinae
Dexamethasone - pharmacology
DNA
DNA - genetics
DNA Restriction Enzymes
DNA, Ribosomal
Genes
Genes - drug effects
Glucocorticoids
Hormonal regulation
Lymphoma, Non-Hodgkin - metabolism
Mesocricetus
Mice
Mice, Inbred BALB C
Nucleic acids
Plasmids
Ribosomal DNA
RNA
RNA Polymerase I - antagonists & inhibitors
RNA, Ribosomal - genetics
Transcription, Genetic - drug effects
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Summary:Cell-free extracts of lymphosarcoma P1798 cell culture lines support faithful initiation upon the cloned mouse DNA encoding rRNA (rDNA) promoter, whereas extracts from cells treated for 16 hr with 0.1μ M dexamethasone cannot. Extracts from both sources transcribe the cloned 5S RNA gene in vitro and mixing experiments further demonstrate that inhibition of transcription of rDNA in vitro is not due to nucleases or inhibitors of transcription present in extracts from glucocorticoid-treated cells. Incubation of extracts from control cells at 45 degrees C for 15 min inactivates RNA polymerase I and abolishes transcription. Activity can be restored by the addition of partially purified RNA polymerase I from control cells and hormone-treated cells. Moreover, extracts from hormone treated cells can be reconstituted by the addition of a partially purified, heat-stable transcription factor from control cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.81.3.718