Purification, crystallization and preliminary X-ray diffraction analysis of crotamine, a myotoxic polypeptide from the Brazilian snake Crotalus durissus terrificus

Crotamine, a highly basic myotoxic polypeptide (molecular mass 4881 Da) isolated from the venom of the Brazilian rattlesnake Crotalus durissus terrificus, causes skeletal muscle contraction and spasms, affects the functioning of voltage‐sensitive sodium channels by inducing sodium influx and possess...

Full description

Saved in:
Bibliographic Details
Published in:Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2012-09, Vol.68 (9), p.1052-1054
Main Authors: Coronado, Mônika A., Georgieva, Dessislava, Buck, Friedrich, Gabdoulkhakov, Azat H., Ullah, Anwar, Spencer, Patrick J., Arni, Raghuvir K., Betzel, Christian
Format: Article
Language:English
Subjects:
Animals
Brazil
Channels
Crotalid Venoms - chemistry
Crotalid Venoms - isolation & purification
Crotalus
Crotalus durissus terrificus
crotamine
Crystallization
Crystallization Communications
Crystallography, X-Ray
Derivatives
Diffraction
Minerals
Molecular structure
myotoxins
Polypeptides
Searching
Sodium
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Crotamine, a highly basic myotoxic polypeptide (molecular mass 4881 Da) isolated from the venom of the Brazilian rattlesnake Crotalus durissus terrificus, causes skeletal muscle contraction and spasms, affects the functioning of voltage‐sensitive sodium channels by inducing sodium influx and possesses antitumour activity, suggesting potential pharmaceutical applications. Crotamine was purified from C. durissus terrificus venom; the crystals diffracted to 1.9 Å resolution and belonged to the orthorhombic space group I212121 or I222, with unit‐cell parameters a = 67.75, b = 74.4, c = 81.01 Å. The self‐rotation function indicated that the asymmetric unit contained three molecules. However, structure determination by molecular replacement using NMR‐determined coordinates was unsuccessful and a search for potential derivatives has been initiated.
ISSN:1744-3091
1744-3091
2053-230X
DOI:10.1107/S1744309112032721